All 14-3-3 beta reagents are produced in house and quality controlled, including 9 14-3-3 beta Antibody, 47 14-3-3 beta Gene, 3 14-3-3 beta IPKit, 3 14-3-3 beta Protein, 2 14-3-3 beta qPCR. All 14-3-3 beta reagents are ready to use.
Recombinant 14-3-3 beta proteins are expressed by E. coli with fusion tags as N-cleavage, N-GST.
14-3-3 betaantibodies are validated with different applications, which are FCM, ELISA, ICC/IF, IF, WB, IP.
14-3-3 betacDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each 14-3-3 beta of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
14-3-3 beta / YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta / YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta / YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta / YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta / YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation.