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人 14-3-3 beta / YWHAB 蛋白 (GST 标签)

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Human YWHAB 蛋白产品信息
分子别称:GW128,HEL-S-1,HS1,KCIP-1,YWHAA
蛋白构建:A DNA sequence encoding the human YWHAB (NP_003395.1) (Met 1-Asn 246) was fused with the GST tag at the N-terminus.
分子种属:Human
表达宿主:E. coli
运输说明:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human YWHAB 蛋白质控检测
纯度:> 96 % as determined by SDS-PAGE
内毒素:Please contact us for more information.
稳定性:Samples are stable for up to twelve months from date of receipt at -70℃
预测N端:Met
分子量:The recombinant human YWHAB/GST chimera consists of 478 amino acids and has a predicted molecular mass of 54.9 kDa. It migrates as an approxiamtely 52 kDa band in SDS-PAGE under reducing conditions.
缓冲液:Lyophilized from sterile 20mM Tris, 150mM NaCl, 0.1mM DTT, 10% glycerol, pH 7.5
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human YWHAB 蛋白使用指导
储存方法:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶条件:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human YWHAB 蛋白SDS-PAGE
Human 14-3-3 beta / YWHAB Protein (GST Tag) SDS-PAGE
Other YWHAB 蛋白 Products
10843-H09E
14-3-3 beta/YWHAB 研究背景

14-3-3 beta / YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta / YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta / YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta / YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta / YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation.

Human 14-3-3 beta/YWHAB 参考资料
  • Tommerup N, et al. (1996) Assignment of the human genes encoding 14,3-3 Eta (YWHAH) to 22q12, 14-3-3 zeta (YWHAZ) to 2p25.1-p25.2, and 14-3-3 beta (YWHAB) to 20q13.1 by in situ hybridization. Genomics. 33(1): 149-50.
  • Jin YH, et al. (2008) Sirt2 interacts with 14-3-3 beta/gamma and down-regulates the activity of p53. Biochem Biophys Res Commun. 368(3): 690-5.
  • Sekimoto T, et al. (2004) 14-3-3 suppresses the nuclear localization of threonine 157-phosphorylated p27(Kip1). EMBO J. 23(9): 1934-42.
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    • Human 14-3-3 beta / YWHAB Protein (GST Tag) SDS-PAGE
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