Anti-14-3-3 eta / YWHAH Antibody

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Anti-14-3-3 eta / YWHAH Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-14-3-3 eta / YWHAH Antibody
经验证的应用
WB,ELISA,IP
交叉反应
Reacts with: Human
特异性
Human 14-3-3 eta / YWHAH
免疫原
Recombinant Human 14-3-3 eta / YWHAH protein (Catalog#10847-H09E)
制备方法
Produced in rabbits immunized with purified, recombinant Human 14-3-3 eta / YWHAH (rh 14-3-3 eta / YWHAH; Catalog#10847-H09E; Q04917; Gly2-Asn246). 14-3-3 eta / YWHAH specific IgG was purified by Human 14-3-3 eta / YWHAH affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-14-3-3 eta / YWHAH Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-14-3-3 eta / YWHAH Antibody (Rabbit Polyclonal 抗体) 图片

Anti-YWHAH rabbit polyclonal antibody at 1:500 dilution

Lane A: U937 Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: Jurkat Whole Cell Lysate

Lane D: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:33 kDa

Observed band size:31 kDa

YWHAH was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

Lane B:0.5 mg Jurkat Whole Cell Lysate

Lane C:0.5 mg k562 Whole Cell Lysate

Lane D:0.5 mg NIH-3T3 Whole Cell Lysate

2 µL anti-YWHAH rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-YWHAH rabbit polyclonal antibody,at 1:2000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 28 kDa

Observed band size: 28 kDa

Anti-14-3-3 eta / YWHAH Antibody: Alternative Names

Anti-YWHA1 Antibody

14-3-3 eta / YWHAH Background Information

YWHAH, the gene encoding the 14-3-3eta isoform, is highly expressed in retinal ganglion cells (RGC). YWHAH is a positional and functional candidate gene for both schizophrenia and bipolar disorder (BP). It is located on chromosome 22q12.3, a region which has been implicated by linkage studies in both BP and schizophrenia.
Full Name
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
References
  • Wakui H, et al. (1997) Interaction of the ligand-activated glucocorticoid receptor with the 14-3-3 eta protein. J Biol Chem. 272(13): 8153-6.
  • Toyooka K, et al. (2002) Isolation and structure of the mouse 14-3-3 eta chain gene and the distribution of 14-3-3 eta mRNA in the mouse brain. Brain Res Mol Brain Res. 100(1-2): 13-20.
  • Kim YS, et al. (2005) Role of 14-3-3 eta as a positive regulator of the glucocorticoid receptor transcriptional activation. Endocrinology. 146(7): 3133-40.
  • Grover D, et al. (2009) Family-based association of YWHAH in psychotic bipolar disorder. Am J Med Genet B Neuropsychiatr Genet. 150B(7): 977-83.
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