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小鼠 14-3-3 sigma / Stratifin / YWHAS 蛋白

  • Mouse 14-3-3 sigma / Stratifin / YWHAS Protein SDS-PAGE
产品数据评论相关产品实验方法
小鼠 YWHAS/SFN 蛋白产品信息
分子别称:Er,Mme1,Ywhas
蛋白构建:A DNA sequence encoding the mouse SFN (NP_061224.2) (Met 1-Ser 248) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminu.
分子种属:Mouse
表达宿主:E. coli
运输说明:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
小鼠 YWHAS/SFN 蛋白质控检测
纯度:> 95 % as determined by SDS-PAGE
内毒素:Please contact us for more information.
稳定性:Samples are stable for up to twelve months from date of receipt at -70℃
预测N端:Gly
分子量:The recombinant mouse SFN consists of 250 amino acids and has a calculated molecular mass of 27.7 kDa. It migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions.
缓冲液:Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 8.0
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
小鼠 YWHAS/SFN 蛋白使用指导
储存方法:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶条件:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
小鼠 YWHAS/SFN 蛋白SDS-PAGE
Mouse 14-3-3 sigma / Stratifin / YWHAS Protein SDS-PAGE

YWHAS/SFN 相关信号通路

    Other YWHAS/SFN 蛋白 Products
    50576-MNCE
    14-3-3 sigma/Stratifin/YWHAS 研究背景

    14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.

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