|IP||1-4 μL/mg of lysate|
|ELISA(Cap)||1:250-1:2000||This antibody will detect Human 2B4 / CD244 in ELISA pair set (Catalog: # SEK10042). In a sandwich ELISA, it can be used as capture antibody when paired with (Catalog: # 10042-R031).|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
2B4 was immunoprecipitated using:
Lane A:0.5 mg Daudi Whole Cell Lysate2 µL anti-2B4 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-2B4 rabbit monoclonal antibody,at 1:200 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 42 kDa
Observed band size: 28 kDa
Anti-2B4 rabbit monoclonal antibody at 1:500 dilution
Lane A: Daudi Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:42 kDa
Observed band size:28 kDa
(We are unsure as to the identity of these extra bands.)
The CD244 antigen, also known as 2B4, is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating NK cell cytotoxicity and cytokine production, which is expressed on all NK cells, a subpopulation of T cells, monocytes and basophils. The 2B4 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor, human 2B4 can effectively activate and enhance NK cell–mediated cytotoxicity, induce secretion of IFN-γ and matrix metalloproteinases (MMPs), as well as NK cell invasiveness. As a cell surface glycoprotein of the Ig-superfamily structurally related to CD2-like molecules such as CD2, CD48, CD58, CD84, Ly-9, and SLAM, 2B4 (CD244) is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. 2B4 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness.
Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Detection: FCM Antibodies
Immune Checkpoint Proteins
Immune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-2B4 / CD244 Antibody||Rabbit||Monoclonal||ELISA(Det)||10042-R031|
|Anti-2B4 / CD244 Antibody||Rabbit||Polyclonal||ELISA||10042-RP01|
|Anti-2B4 / CD244 Antibody||Rabbit||Monoclonal||ELISA||10042-R020|
|Anti-2B4 / CD244 Antibody||Mouse||Monoclonal||ELISA||10042-MM02|
|Anti-2B4 / CD244 Antibody||Rabbit||Polyclonal||ELISA||10042-RP02|