Anti-4-1BBL Antibody

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Anti-4-1BBL Antibody (Rabbit Monoclonal 抗体) 产品信息

产品名称
Anti-4-1BBL Antibody
经验证的应用
ELISA,FCM,ICC/IF
交叉反应
Reacts with: Mouse
特异性
Mouse 4-1BBL
免疫原
Recombinant Mouse TNFSF9 protein (Catalog#50067-M07H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse TNFSF9 / 4-1BBL (rM TNFSF9; Catalog#50067-M07H; Arg 104-Glu 309; NP_033430.1).
来源
Monoclonal Rabbit IgG Clone #014
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-4-1BBL Antibody (Rabbit Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
ELISA 1:1000-1:2000
ICC-IF 1:50-1:1000
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-4-1BBL Antibody (Rabbit Monoclonal 抗体) 图片

Confocal immunofluorescence analysis of Mouse TNFSF9 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse TNFSF9 monoclonal antibody (1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Flow cytometric analysis of Mouse TNFSF9 expression on Raw264.7 cells. Cells were stained with purified anti-Mouse TNFSF9, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Anti-4-1BBL Antibody: 别称

Anti-4-1BB-L Antibody; Anti-4-1BBL Antibody; Anti-AI848817 Antibody; Anti-Cd137l Antibody; Anti-Ly63l Antibody

4-1BBL 背景信息

4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. It is shown to be a type II surface glycoprotein belonging to the TNF superfamily. Expression of 4-1BBL is restricted to APCs, such as dendritic cells, macrophages, and activated B cells. Members of the TNF-TNF receptor superfamily have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis. Several studies have reported that 4-1BBL/4-1BB interaction provided a co-stimulatory signal to T cells, and increased T cell proliferation and cytokines production. In additon, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.
全称
tumor necrosis factor (ligand) superfamily, member 9
参考文献
  • Cheung CT, (2007) Neutralizing anti-4-1BBL treatment improves cardiac function in viral myocarditis. Lab Invest. 87(7): 651-61.
  • Wang C, et al. (2009) Immune regulation by 4-1BB and 4-1BBL: complexities and challenges. Immunol Rev. 229(1): 192-215.
  • Habib-Agahi M, et al. (2009) 4-1BBL costimulation retrieves CD28 expression in activated T cells. Cell Immunol. 256(1-2): 39-46.
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