Anti-4EBP1 Antibody

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Anti-4EBP1 Antibody (Rabbit Monoclonal 抗体) 产品信息

产品名称
Anti-4EBP1 Antibody
经验证的应用
ICC/IF,IF
交叉反应
Reacts with: Human
特异性
Human 4EBP1
免疫原
Recombinant Human 4E-BP1 / EIF4EBP1 Protein (Catalog#10022-H07E)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human 4E-BP1 / EIF4EBP1 (rh 4E-BP1 / EIF4EBP1; Catalog#10022-H07E; NP_004086.1; Ser2-Ile118).
来源
Monoclonal Rabbit IgG Clone #046
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-4EBP1 Antibody (Rabbit Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
ICC-IF 1:20-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-4EBP1 Antibody (Rabbit Monoclonal 抗体) 图片

Immunofluorescence staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human 4EBP1 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus and cytoplasm.

Anti-4EBP1 Antibody: 别称

Anti-4E-BP1 Antibody; Anti-4EBP1 Antibody; Anti-BP-1 Antibody; Anti-PHAS-I Antibody

4EBP1 背景信息

The translational suppressor eIF4E binding protein-1, 4E-BP1 functions as a key regulator in cellular growth, differentiation, apoptosis and survival. The Eif4ebp1 gene, encoding 4E-BP1, is a direct target of a transcription factor activating transcription factor-4 (ATF4), a master regulator of gene expression in stress responses. 4E-BP1 is characterized by its capacity to bind specifically to eIF4E and inhibit its interaction with eIF4G. Phosphorylation of 4E-BP1 regulates eIF4E availability, and therefore, cap-dependent translation, in cell stress. Binding of eIF4E to eIF4G is inhibited in a competitive manner by 4E-BP1. Phosphorylation of 4E-BP1 decreases the affinity of this protein for eIF4E, thus favouring the binding of eIF4G and enhancing translation. 4E-BP1 is important for beta-cell survival under endoplasmic reticulum (ER) stress. 4E-BP1 mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. Recently, 4E-BP1 was found to be a key factor, which converges several oncogenic signals, phosphorylates the molecules, and drives the downstream proliferative signals. Recent studies showed that high expression of phosphorylated 4E-BP-1 (p-4E-BP1) is associated with poor prognosis, tumor progression, or nodal metastasis in different human cancers.
全称
eukaryotic translation initiation factor 4E binding protein 1
参考文献
  • Azar R, et al. (2008) Phosphatidylinositol 3-kinase-dependent transcriptional silencing of the translational repressor 4E-BP1. Cell Mol Life Sci. 65(19): 3110-7.
  • Tominaga R, et al. (2010) The JNK pathway modulates expression and phosphorylation of 4E-BP1 in MIN6 pancreatic beta-cells under oxidative stress conditions. Cell Biochem Funct. 28(5): 387-93.
  • Ayuso MI, et al. (2010) New hierarchical phosphorylation pathway of the translational repressor eIF4E-binding protein 1 (4E-BP1) in ischemia-reperfusion stress. J Biol Chem. 285(45): 34355-63.
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