Anti-4EBP1 Antibody

Cat: 10022-MM05

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Anti-4EBP1 Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-4EBP1 Antibody
Validated applications
WB,ELISA,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human 4EBP1
No cross-reactivity in ELISA with E.coli cell lysate
Immunogen
Recombinant Human 4E-BP1 / EIF4EBP1 Protein (Catalog#10022-H07E)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 4E-BP1 / EIF4EBP1 (rh 4E-BP1 / EIF4EBP1; Catalog#10022-H07E; Ser 2-Ile 118; NP_004086.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography .
Source
Monoclonal Mouse IgG1 Clone #5F3G9
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-4EBP1 Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
ICC-IF 1:100-1:500  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-4EBP1 Antibody (Mouse Monoclonal antibody) Images

Human 4E-BP1/EIF4EBP1 Immunofluorescence(IF) 3023
Immunofluorescence staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human 4EBP1 monoclonal antibody (1:300) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm.
Human 4E-BP1/EIF4EBP1 Western blot (WB) 6828

Anti-4EBP1 mouse monoclonal antibody at 1:500 dilution

Lane A: A431 Whole Cell Lysate

Lane B: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:13 kDa

Observed band size:13 kDa

Anti-4EBP1 Antibody Alternative Names

Anti-4E-BP1 Antibody;Anti-4EBP1 Antibody;Anti-BP-1 Antibody;Anti-PHAS-I Antibody

4EBP1 Background Information

The translational suppressor eIF4E binding protein-1, 4E-BP1 functions as a key regulator in cellular growth, differentiation, apoptosis and survival. The Eif4ebp1 gene, encoding 4E-BP1, is a direct target of a transcription factor activating transcription factor-4 (ATF4), a master regulator of gene expression in stress responses. 4E-BP1 is characterized by its capacity to bind specifically to eIF4E and inhibit its interaction with eIF4G. Phosphorylation of 4E-BP1 regulates eIF4E availability, and therefore, cap-dependent translation, in cell stress. Binding of eIF4E to eIF4G is inhibited in a competitive manner by 4E-BP1. Phosphorylation of 4E-BP1 decreases the affinity of this protein for eIF4E, thus favouring the binding of eIF4G and enhancing translation. 4E-BP1 is important for beta-cell survival under endoplasmic reticulum (ER) stress. 4E-BP1 mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. Recently, 4E-BP1 was found to be a key factor, which converges several oncogenic signals, phosphorylates the molecules, and drives the downstream proliferative signals. Recent studies showed that high expression of phosphorylated 4E-BP-1 (p-4E-BP1) is associated with poor prognosis, tumor progression, or nodal metastasis in different human cancers.

Full Name
eukaryotic translation initiation factor 4E binding protein 1
References
  • Azar R, et al. (2008) Phosphatidylinositol 3-kinase-dependent transcriptional silencing of the translational repressor 4E-BP1. Cell Mol Life Sci. 65(19): 3110-7.
  • Tominaga R, et al. (2010) The JNK pathway modulates expression and phosphorylation of 4E-BP1 in MIN6 pancreatic beta-cells under oxidative stress conditions. Cell Biochem Funct. 28(5): 387-93.
  • Ayuso MI, et al. (2010) New hierarchical phosphorylation pathway of the translational repressor eIF4E-binding protein 1 (4E-BP1) in ischemia-reperfusion stress. J Biol Chem. 285(45): 34355-63.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-4EBP1 Antibody Rabbit Monoclonal WB,ELISA 10022-R058
    Anti-4EBP1 Antibody Rabbit Monoclonal ICC/IF,IF 10022-R007
    Anti-4EBP1 Antibody Rabbit Polyclonal ELISA 10022-RP01
    Anti-4EBP1 Antibody Mouse Monoclonal ICC/IF,IF 10022-MM01T
    Anti-4EBP1 Antibody Mouse Monoclonal FCM 10022-MM04
    Anti-4EBP1 Antibody (FITC) Mouse Monoclonal FCM 10022-MM04-F
    Anti-4EBP1 Antibody (PE) Mouse Monoclonal FCM 10022-MM04-P
    Anti-4EBP1 Antibody Rabbit Polyclonal ELISA 10022-T16
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