To get the best Western Blotting performance from your Western blotting antibody, please use the positive control lysate recommended on our Technical Data Sheets. The positive control will help you duplicate our established results in initial experiments by precisely following our established protocols.
We recommend these lysates to be stored at -20°C for long-term storage. Lysates are extremely stable since they are cell preparations in which all enzymes are denatured and inactivated. We have done extensive research and confirmed stability under different conditions. For example, the integrity and quality of most lysates were not affected by repeated freeze-thaw cycles, or storage at 25°C for up to 12 weeks. The lysates, however, started to show degradation by four weeks at 37°C. There may, however, be some slight variation in stability depending on the source of the lysate so we do strongly recommend that they are stored at -20°C.