AADAC/arylacetamide deacetylase cDNA ORF Clone, Human, C-DDK (Flag®) tag

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AADAC/arylacetamide deacetylase cDNA ORF Clone, Human, C-DDK (Flag®) tag: 产品信息

基因
种属
Human
NCBI 参考序列号
基因长度
1200 bp
序列特征
Identical with the Gene Bank Ref. ID sequence.
产品特征
Full length Clone DNA of Human arylacetamide deacetylase (esterase) with Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV2-FLAG
限制性酶切位点
KpnI + XhoI (5.4kb + 1.25kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

AADAC/arylacetamide deacetylase cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

AADAC/arylacetamide deacetylase cDNA ORF Clone, Human, C-DDK (Flag®) tag: 验证图片

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

AADAC/arylacetamide deacetylase cDNA ORF Clone, Human, C-DDK (Flag®) tag: 别称

CES5A1 cDNA ORF Clone, Human; DAC cDNA ORF Clone, Human
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