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小鼠 AADAC/arylacetamide deacetylase 基因ORF全长cDNA(克隆载体)

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Mouse AADAC cDNA Clone产品信息
NCBI注册码:NM_023383.1
参考序列ORF长度:1197bp
cDNA基因描述:Full length Clone DNA of Mus musculus arylacetamide deacetylase (esterase).
分子别称:Aada, AI265437, 5033417E09Rik, Aadac
分子种属:Mouse
载体:pMD18-T Simple Vector
质粒:pMD-mAADAC
限制性酶切位点:
Tag序列:
序列信息:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 168T>C, 270C>T, 570T>G and 1086G>T not causing the amino acid variation; 301G>A(A101T).
测序引物:M13-47 and RV-M
启动子:
Application:
抗生素(大肠杆菌):Ampicilin
抗生素(哺乳动物细胞):
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
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货号: MG50243-M
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