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小鼠 AAMP 基因ORF全长cDNA(克隆载体)

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    小鼠 AAMP cDNA Clone产品信息
    NCBI注册码:NM_146110.2
    参考序列ORF长度:1308
    cDNA基因描述:ORF Clone of Mus musculus angio-associated migratory protein DNA.
    分子别称:Aamp-rs, AU040907, MGC30660, Aamp
    分子种属:Mouse
    载体:pMD18-T Simple Vector
    限制性酶切位点:
    Tag序列:
    序列信息:Identical with the Gene Bank Ref. ID sequence except for two point mutations: 1-2 AT/TA not causing the amino acid variation.
    测序引物:( We provide with AAMP qPCR primers for gene expression analysis, MP200249 )
    抗生素(大肠杆菌):Ampicillin
    运输方式:Each tube contains approximately 10 μg of lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at ambient temperature for three months.
    pMD18-T Simple Vector Information

    pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

    pMD18-T Simple Usage Suggestion

    The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

    Vector Sequence Download
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