快速订购

Text Size:AAA

AARS / alanyl-tRNA synthetase 抗体, 兔多抗, 抗原亲和纯化

  • AARS / alanyl-tRNA synthetase Antibody, Rabbit PAb, Antigen Affinity Purified
  • AARS / alanyl-tRNA synthetase Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
  • AARS / alanyl-tRNA synthetase Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
  • AARS / alanyl-tRNA synthetase Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
  • AARS / alanyl-tRNA synthetase Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
产品数据评论相关产品实验方法
小鼠 AARS 抗体产品信息
免疫原:Recombinant Mouse AARS / alanyl-tRNA synthetase Protein (Catalog#50384-M08B)
克隆号:
Ig 类型:Rabbit IgG
浓度:
内毒素:
缓冲液:0.2 μm filtered solution in PBS
抗体制备方法:Produced in rabbits immunized with purified, recombinant Mouse AARS / alanyl-tRNA synthetase (rM AARS / alanyl-tRNA synthetase; Catalog#50384-M08B; Q8BGQ7; Met1-Asn968). AARS / alanyl-tRNA synthetase specific IgG was purified by Mouse AARS / alanyl-tRNA synthetase affinity chromatography.
小鼠 AARS 抗体WB实验图片
小鼠 AARS 抗体IHC实验图片
AARS / alanyl-tRNA synthetase Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[点击查看大图]
Caption:
Immunochemical staining of mouse AARS in mouse kidney with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
AARS / alanyl-tRNA synthetase Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[点击查看大图]
Caption:
Immunochemical staining of mouse AARS in mouse stomach with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
小鼠 AARS 抗体IF实验图片
AARS / alanyl-tRNA synthetase Antibody, Rabbit PAb, Antigen Affinity Purified
[点击查看大图]
Caption:
Immunofluorescence staining of AARS in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-mouse AARS polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
小鼠 AARS 抗体IP实验图片
Other AARS 抗体 Products

Anti-Histone H3 rabbit monoclonal antibody at 1:200 dilution

Lane A: NIH3T3 Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:15 kDa

Observed band size:17 kDa

AARS / alanyl-tRNA synthetase 研究背景

Alanyl-tRNA synthetase (AARS) belongs to the family of ligases, specifically those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis. Alanyl-tRNA synthetase (AlaRS) catalyzes synthesis of Ala-tRNA (Ala) and hydrolysis of mis-acylated Ser- and Gly-tRNA (Ala) at 2 different catalytic sites. Their role is not confined to catalyze the attachment of amino acids to transfer RNAs and thereby establish the rules of genetic code by virtue of matching the nucleotide triplet of anticodon with cognate amino acid. Under apoptotic conditions in cell culture, the full-length enzyme is secreted, and the two cytokine activities can be generated by leukocyte elastase, an extracellular protease. Secretion of this tRNA synthetase may contribute to apoptosis both by arresting translation and producing needed cytokines. This protein could be an attractive target of drugs against bacterial, fungal and parasitic infections. 

小鼠 AARS / alanyl-tRNA synthetase 参考资料
  • Wakasugi K, et al. (1999) Two Distinct Cytokines Released from a Human Aminoacyl-tRNA Synthetase. Science. 284 (5411): 147-51.
  • Sokabe M, et al. (2009) The structure of alanyl-tRNA synthetase with editing domain. Proc Natl Acad Sci . 106 (27): 11028-33.
  • Skupinska M, et al. (2009) AARS--the etiological factor and the attractive target of many disorders. Postepy Biochem. 55 (4): 373-84.
  • Product nameProduct name
    Size / Price
    货号: 50384-T60-200
    目录价: 
    单价:      (You Save: )

    Datasheet & Documentation

    产品数据可能随批次变化,最新数据请详见随货说明书。
    请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”