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小鼠 AARS / alanyl-tRNA synthetase 蛋白 (His 标签)

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Mouse AARS 蛋白产品信息
分子别称:AI316495,C76919,sti
蛋白构建:A DNA sequence encoding the mouse AARS (Q8BGQ7) (Met 1-Asn 968) was fused with a polyhistidine tag at the C-terminus.
分子种属:Mouse
表达宿主:Baculovirus-Insect Cells
运输说明:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Mouse AARS 蛋白质控检测
纯度:> 88 % as determined by SDS-PAGE
内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
稳定性:Samples are stable for up to twelve months from date of receipt at -70℃
预测N端:Met 1
分子量:The secreted recombinant mouse AARS consists of 978 amino acids and has a calculated molecular mass of 108.3 kDa. The recombinant protein migrates as an approximately 105 kDa band in SDS-PAGE under reducing conditions.
缓冲液:Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Mouse AARS 蛋白使用指导
储存方法:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶条件:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Mouse AARS 蛋白SDS-PAGE
Mouse AARS / alanyl-tRNA synthetase Protein (His Tag) SDS-PAGE
Other AARS 蛋白 Products
50384-M08B
AARS / alanyl-tRNA synthetase 研究背景

Alanyl-tRNA synthetase (AARS) belongs to the family of ligases, specifically those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis. Alanyl-tRNA synthetase (AlaRS) catalyzes synthesis of Ala-tRNA (Ala) and hydrolysis of mis-acylated Ser- and Gly-tRNA (Ala) at 2 different catalytic sites. Their role is not confined to catalyze the attachment of amino acids to transfer RNAs and thereby establish the rules of genetic code by virtue of matching the nucleotide triplet of anticodon with cognate amino acid. Under apoptotic conditions in cell culture, the full-length enzyme is secreted, and the two cytokine activities can be generated by leukocyte elastase, an extracellular protease. Secretion of this tRNA synthetase may contribute to apoptosis both by arresting translation and producing needed cytokines. This protein could be an attractive target of drugs against bacterial, fungal and parasitic infections. 

Mouse AARS / alanyl-tRNA synthetase 参考资料
  • Wakasugi K, et al. (1999) Two Distinct Cytokines Released from a Human Aminoacyl-tRNA Synthetase. Science. 284 (5411): 147-51.
  • Sokabe M, et al. (2009) The structure of alanyl-tRNA synthetase with editing domain. Proc Natl Acad Sci . 106 (27): 11028-33.
  • Skupinska M, et al. (2009) AARS--the etiological factor and the attractive target of many disorders. Postepy Biochem. 55 (4): 373-84.
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    货号: 50384-M08B-100
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    • Mouse AARS / alanyl-tRNA synthetase Protein (His Tag) SDS-PAGE
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