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ABHD14B 抗体, 兔多抗, 抗原亲和纯化

  • ABHD14B Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
  • ABHD14B Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
产品数据评论相关产品实验方法
 ABHD14B 抗体产品信息
免疫原:Recombinant Human ABHD14B protein (Catalog#13913-H07E)
克隆号:
Ig 类型:Rabbit IgG
浓度:
内毒素:
缓冲液:0.2 μm filtered solution in PBS
抗体制备方法:Produced in rabbits immunized with purified, recombinant Human ABHD14B (rh ABHD14B; Catalog#13913-H07E; Q96IU4-1; Met1-Gln210). ABHD14B specific IgG was purified by Human ABHD14B affinity chromatography.
 ABHD14B 抗体IHC实验图片
Other ABHD14B 抗体 Products

Anti-Histone H3 rabbit monoclonal antibody at 1:200 dilution

Lane A: NIH3T3 Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:15 kDa

Observed band size:17 kDa

ABHD14B 研究背景

ABHD14B belongs to the AB hydrolase superfamily, ABHD14 family. It can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, heart, placenta, lung, liver, skeletal muscle, pancreas and kidney. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro) and may interact with TAF1. It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. ABHD14B contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing gene subfamily is comprised of 15 mostly uncharacterized members.

 ABHD14B 参考资料
  • Mehrle A, et al. (2006) The LIFEdb database in 2006. Nucleic Acids Res. 34:D415-8.
  • Wan D, et al. (2004) Large-scale cDNA transfection screening for genes related to cancer development and progression. Proc Natl Acad Sci. 101(44):15724-9.
  • Wiemann S, et al. (2004) From ORFeome to biology: a functional genomics pipeline. Genome Res. 14 (10B):2136-44.
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    货号: 13913-T24-200
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    Datasheet & Documentation

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