All ABHD14B reagents are produced in house and quality controlled, including 4 ABHD14B Antibody, 15 ABHD14B Gene, 1 ABHD14B IPKit, 1 ABHD14B Protein, 1 ABHD14B qPCR. All ABHD14B reagents are ready to use.
Recombinant ABHD14B proteins are expressed by E. coli with fusion tags as N-His.
ABHD14Bantibodies are validated with different applications, which are WB, IP, ELISA, IHC-P, ICC/IF, IF.
ABHD14BcDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each ABHD14B of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
ABHD14B belongs to the AB hydrolase superfamily, ABHD14 family. It can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, heart, placenta, lung, liver, skeletal muscle, pancreas and kidney. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro) and may interact with TAF1. It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. ABHD14B contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing gene subfamily is comprised of 15 mostly uncharacterized members.