Anti-Adenylosuccinate Lyase Antibody

Cat: 11287-R007

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Anti-Adenylosuccinate Lyase Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-Adenylosuccinate Lyase Antibody
Validated applications
WB,IHC-P,ICC/IF,IF,IP
Species reactivity
Reacts with: Human
Specificity
Human Adenylosuccinate Lyase
Immunogen
Recombinant Human ADSL / Adenylosuccinate Lyase protein (Catalog#11287-H07E)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human ADSL / Adenylosuccinate Lyase (rh ADSL / Adenylosuccinate Lyase; Catalog#11287-H07E; P30566-1; Met1-Leu484).
Source
Monoclonal Rabbit IgG Clone #007
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-Adenylosuccinate Lyase Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution Notes
WB 1:500-1:2000  
IHC-P 1:250-1:1000  
ICC-IF 1:20-1:100  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Adenylosuccinate Lyase Antibody (Rabbit Monoclonal antibody) Images

Human ADSL / Adenylosuccinate Lyase Immunohistochemistry(IHC) 4743
Immunochemical staining of human ADSL in human thyroid with rabbit monoclonal antibody (1:500, formalin-fixed paraffin embedded sections).
Human ADSL / Adenylosuccinate Lyase Immunohistochemistry(IHC) 4744
Immunochemical staining of human ADSL in human small intestine with rabbit monoclonal antibody (1:500, formalin-fixed paraffin embedded sections).
Human ADSL / Adenylosuccinate Lyase Immunohistochemistry(IHC) 4745
Immunochemical staining of human ADSL in human ovarian cancer with rabbit monoclonal antibody (1:500, formalin-fixed paraffin embedded sections).
Human ASGPR1  Immunoprecipitation(IP) 8018

ADSL was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-ADSL rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-ADSL rabbit monoclonal antibody,at 1:200 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 50 kDa

Observed band size: 50 kDa

Human ADSL / Adenylosuccinate Lyase Western blot (WB) 13384

Anti-ADSL rabbit monoclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lane C: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:55 kDa

Observed band size:55 kDa

Human ADSL / Adenylosuccinate Lyase Immunofluorescence(IF) 16033
Immunofluorescence staining of ADSL in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human ADSL monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).

Anti-Adenylosuccinate Lyase Antibody Alternative Names

Anti-AMPS Antibody;Anti-ASASE Antibody;Anti-ASL Antibody

Adenylosuccinate Lyase Background Information

Adenylosuccinate lyase, also known as adenylosuccinase, ADSL or ASL, is an enzyme implicated in the reaction of adenylosuccinat converting to AMP and fumarate as part of the purine nucleotide cycle. The two substates of adenylosuccinate lyase (ADSL) are dephosphorylated derivatives of SAICA ribotide (SAICAR) and adenylosuccinate (S-AMP), which catalyzes an important reaction in the de novo pathway of purine biosynthesis. ADSL catalyzes two distinct reactions in the synthesis of purine nucleotides, both of which involve the _-elimination of fumarate to produce either aminoimidazole carboxamide ribotide from SAICAR or AMP from S-AMP. The Adenylosuccinate lyase deficiency is a rare autosomal recessive metabolic disorder characterized by the present of SAICA riboside and succinyladenosine (S-Ado). ADSL defect in different patients is often caused by different mutations to the enzyme.

Full Name
adenylosuccinate lyase
References
  • Nassogne M, et al. (2000) Adenylosuccinase deficiency: an unusual cause of early-onset epilepsy associated with acquired microcephaly. Brain and development. 22 (6): 383-6.
  • Sivendran S, et al. (2004) Two novel mutant human adenylosuccinate lyases (ASLs) associated with autism and characterization of the equivalent mutant Bacillus subtilis ASL. J Biol Chem. 279 (51): 53789-97.
  • Lee TT, et al. (1999) His68 and His141 are critical contributors to the intersubunit catalytic site of adenylosuccinate lyase of Bacillus subtilis. Biochemistry. 38 (1): 22-32.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Adenylosuccinate Lyase Antibody Rabbit Polyclonal ELISA 11287-RP01
    Anti-Adenylosuccinate Lyase Antibody Mouse Monoclonal ICC/IF,IF 11287-MM05
    Anti-Adenylosuccinate Lyase Antibody Rabbit Polyclonal WB,ELISA 11287-RP02
    Anti-Adenylosuccinate Lyase Antibody Rabbit Monoclonal ELISA 11287-R001
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