|IP||1-4 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
ADSL was immunoprecipitated using:
Lane A:0.5 mg HepG2 Whole Cell Lysate2 µL anti-ADSL rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-ADSL rabbit monoclonal antibody,at 1:200 dilutionSecondary antibody:
Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.
Performed under reducing conditions.Predicted band size: 50 kDa
Observed band size: 50 kDa
Anti-ADSL rabbit monoclonal antibody at 1:500 dilution
Lane A: HepG2 Whole Cell Lysate
Lane B: Jurkat Whole Cell Lysate
Lane C: Hela Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:55 kDa
Observed band size:55 kDa
Adenylosuccinate lyase, also known as adenylosuccinase, ADSL or ASL, is an enzyme implicated in the reaction of adenylosuccinat converting to AMP and fumarate as part of the purine nucleotide cycle. The two substates of adenylosuccinate lyase (ADSL) are dephosphorylated derivatives of SAICA ribotide (SAICAR) and adenylosuccinate (S-AMP), which catalyzes an important reaction in the de novo pathway of purine biosynthesis. ADSL catalyzes two distinct reactions in the synthesis of purine nucleotides, both of which involve the _-elimination of fumarate to produce either aminoimidazole carboxamide ribotide from SAICAR or AMP from S-AMP. The Adenylosuccinate lyase deficiency is a rare autosomal recessive metabolic disorder characterized by the present of SAICA riboside and succinyladenosine (S-Ado). ADSL defect in different patients is often caused by different mutations to the enzyme.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-Adenylosuccinate Lyase Antibody||Rabbit||Polyclonal||ELISA||11287-RP01|
|Anti-Adenylosuccinate Lyase Antibody||Mouse||Monoclonal||ICC/IF,IF||11287-MM05|
|Anti-Adenylosuccinate Lyase Antibody||Rabbit||Polyclonal||WB,ELISA||11287-RP02|
|Anti-Adenylosuccinate Lyase Antibody||Rabbit||Monoclonal||ELISA||11287-R001|