Bone Alkaline Phosphatase Protein, Mouse, Recombinant (Fc Tag)

Cat: 51134-M02H

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Bone Alkaline Phosphatase Protein, Mouse, Recombinant (Fc Tag) 产品信息

Purity
> 90 % as determined by SDS-PAGE.
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the mouse Alpl (BAH03518.1) (Met1-Ser502) was expressed with the Fc region of human IgG1 at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Phe 18
Molecule Mass
The recombinant mouse Alpl consists 723 amino acids and predicts a molecular mass of 80.3 kDa.
Formulation
Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Bone Alkaline Phosphatase Protein, Mouse, Recombinant (Fc Tag) in SDS-PAGE

Mouse Alkaline Phosphatase / ALPL Protein 12115

Bone Alkaline Phosphatase Protein, Mouse, Recombinant (Fc Tag) Alternative Names

Akp-2 Protein, Mouse;Akp2 Protein, Mouse;APTNAP Protein, Mouse;TNAP Protein, Mouse;TNSALP Protein, Mouse

Bone Alkaline Phosphatase Background Information

Alkaline phosphatase (ALPL) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. Alkaline phosphatases (APs) are ubiquitous in many species, from bacteria to human. Four genes encode AP isoenzymes in humans and rodents. Three AP genes are expressed in a tissue-specific manner (i.e., placental, embryonic, and intestinal AP isoenzymes). Expression of the fourth AP gene is nonspecific to a single tissue and is especially abundant in bone, liver, and kidney. This isoenzyme is also called tissue-nonspecific alkaline phosphatase (TNAP). The enzyme tissue non-specific alkaline phosphatase (TNAP) belongs to the ectophosphatase family. TNAP is present in large amounts in bone in which it plays a role in mineralization.

Full Name
alkaline phosphatase, liver/bone/kidney
References
  • Brun-Heath I, et al. (2011) Differential expression of the bone and the liver tissue non-specific alkaline phosphatase isoforms in brain tissues. Cell Tissue Res. 343(3): 521-36.
  • Whyte MP, et al. (1995) Alkaline phosphatase: placental and tissue-non-specific isoenzymes hydrolyze phosphoethanolamine, inorganic pyrophosphate, and pyridoxal 5-phosphate. J Clin Invest. 95: 1440-5.
  • Whyte MP. (1994) Hypophosphatasia and the role of alkaline phosphatase in skeletal mineralization. Endocrinol Rev. 4: 439-61.
  • Weinreb M, et al. (1990) Different pattern of alkaline phosphatase, osteopontin and osteocalcin expression in developing rat bone visualized by in situ hybridization. J Bone Miner Res. 5: 831-42.
  • Other Bone Alkaline Phosphatase Proteins

    Mouse Bone Alkaline Phosphatase Proteins

    Product Name Cat.No.
    Bone Alkaline Phosphatase Protein, Mouse, Recombinant (His Tag) 51134-M08H

    Human Bone Alkaline Phosphatase Proteins

    Product Name Cat.No.
    Bone Alkaline Phosphatase Protein, Human, Recombinant (His Tag) 10440-H08H
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