Anti-E-tag Antibody

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Anti-E-tag Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-E-tag Antibody
经验证的应用
WB,ICC/IF,IP
特异性
E-tag
免疫原
A synthetic peptide corresponding to the E-tag sequence (GAPVPYPDPLEPR).
制备方法
Produced in rabbits immunized with A synthetic peptide corresponding to the E-tag sequence (GAPVPYPDPLEPR), and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-E-tag Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ICC-IF 1:300-1:10000
IP 0.5-2 μg/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-E-tag Antibody (Rabbit Polyclonal 抗体) 图片

Immunofluorescence staining of E-Tag in Hela cells, transfected with pSTEP2-Etag-GFERh (Figure A), pSTEP2-his-Etag-GFER (Figure B), pSTEP2-his-GFER-Etag (Figure C), pSTEP2-Etag-GSTT2h (Figure D),pSTEP2-his-Etag-GSTT2 (Figure E) and pSTEP2-his-GSTT2-Etag (Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-E-Tag polyclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).

E-tag was immunoprecipitated using:

Lane A:0.5 mg E tag-1M-125D-His© Whole Cell Lysate

Lane B:0.5 mg His-E tag-1M-125D© Whole Cell Lysate

Lane C:0.5 mg His-1M-125D-E tag© Whole Cell Lysate

Lane D:0.5 mg E tag-1M-244P-His© Whole Cell Lysate

Lane E:0.5 mg Psetp2 Whole Cell Lysate

2 µL anti-E-tag rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-E-tag rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Anti-E Tag rabbit polyclonal antibody at 1:1000 dilution.

Lane A: Etag-GFERh(C) transfected 293 cell lysate at 2ug

Lane B: his-Etag-GFER(C) transfected 293 cell lysate at 2ug

Lane C: his-GFER-Etag(C) transfected 293 cell lysate at 2ug

Lane D: Etag-GSTT2h(C) transfected 293 cell lysate at 10ug

Lane E: his-GSTT2-Etag(C) transfected 293 cell lysate at 10ug

Lane F: his-Etag-GSTT2(C) transfected 293 cell lysate at 20ug

Lane G: Nagetive control at 10ug

Secondary

Dylight 800-labeled Antibody To Rabbit IgG (H+L) at 1/5000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

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