Anti-H7N9 Hemagglutinin / HA Antibody, Rabbit PAb, Antigen Affinity Purified

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Anti-H7N9 Hemagglutinin / HA Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-H7N9 Hemagglutinin / HA Antibody, Rabbit PAb, Antigen Affinity Purified
经验证的应用
WB,ELISA,IHC-P,FCM,ICC/IF,IF,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
应用说明
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
特异性
Influenza Hemagglutinin / HA
Has cross-reactivity in ELISA with
H7N9 (A/Anhui/1/2013) HA
免疫原
Recombinant Influenza A H7N9 (A/Shanghai/1/2013) HA protein (Catalog#40104-V08B)
制备方法
Produced in rabbits immunized with purified, recombinant Influenza A H7N9 (A/Shanghai/1/2013) HA (Catalog#40104-V08B; Met1-Val524). HA specific IgG was purified by Influenza A H7N9 (A/Shanghai/1/2013) HA affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-H7N9 Hemagglutinin / HA Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:5000-1:10000. Using a DAB detection system, the detection limits for H7N9 (A/Anhui/1/2013) HA and H7N9 (A/Shanghai/1/2013) HA are approximately 10 ng/lane under reducing conditions.
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Hemagglutinin / HA 背景信息

The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
全称
Harvey rat sarcoma viral oncogene homolog
参考文献
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). ""Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin"". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). ""Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses"". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). ""Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential"". Avian Dis. 40 (2): 425–37
  • Hemagglutinin stalk domain from H5N1 strain as a potentially universal antigen.
    Author
    K Uranowska, J Tyborowska, A Jurek…
    Year
    2013
    Journal
    Acta biochimica polonica
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