Anti-Myc tag Antibody

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Anti-Myc tag Antibody (Mouse Monoclonal 抗体) 产品信息

产品名称
Anti-Myc tag Antibody
经验证的应用
WB,IP
特异性
Myc tag
免疫原
A synthetic Myc tag peptide conjugated to KLH
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic Myc tag peptide conjugated to KLH. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG1 Clone #08
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Myc tag Antibody (Mouse Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1/5000-1/10000
IP IP:1-5μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Myc tag Antibody (Mouse Monoclonal 抗体) 图片

Anti-Myc Tag mouse monoclonal antibody at 1:5000 dilution.

Lane A: GST-myc(Recombinant protein) (5ng)

Lane B: GST-myc(Recombinant protein) (1ng)

Lane C: myc-GST(Recombinant protein) (100ng)

Lane D: myc-GST(Recombinant protein) (20ng)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-Myc Tag mouse monoclonal antibody at 1:5000 dilution.

Lane A: HA-mFABP4-myc transfected 293 cell lysate (10ug)

Lane B: myc-ARG1-HA transfected 293 cell lysate (5ug)

Lane C: HA-ARG1-myc transfected 293 cell lysate (10ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution

Developed using the Odyssey technique.

Performed under reducing conditions.

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