Anti-BMP-2 Antibody

Cat: 10426-R110
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All BMP-2 Reagents
Anti-BMP-2 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-BMP-2 Antibody
Validated applications
ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human BMP-2
Immunogen
Recombinant Human BMP-2 protein (Catalog#10426-HNAE)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human BMP-2 (rh BMP-2; Catalog#10426-HNAE; NP_001191.1; Gln283-Arg396).
Source
Monoclonal Rabbit IgG Clone #110
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-BMP-2 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
ICC-IF 1:20-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-BMP-2 Antibody (Rabbit Monoclonal antibody) Images
Human BMP-2 Immunofluorescence(IF) 2999
Immunofluorescence staining of Human BMP2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human BMP2 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Anti-BMP-2 Antibody Alternative Names
Anti-BDA2 Antibody;Anti-BDA2A Antibody;Anti-BMP-2 Antibody;Anti-BMP2A Antibody
BMP-2 Background Information

BMP-2 protein, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. BMP-2 protein is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. BMP-2 and BMP-7 are osteogenic BMPs that have been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2, BMP-4 and BMP-7 are known to be of major importance in bone formation and repair. In cancerous tissues BMP-2 protein may play an important role in the progression of glioma.

Full Name
bone morphogenetic protein 2
References
  • Jiao X, et al. (2007) Heparan sulfate proteoglycans (HSPGs) modulate BMP-2 osteogenic bioactivity in C2C12 cells. J Biol Chem. 282(2):1080-6.
  • Michon F, et al. (2008) BMP-2 and BMP-7 play antagonistic roles in feather induction. Development 135 (16):2797-805.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-BMP-2 Antibody Mouse Monoclonal ELISA(Det) 10426-MM03
    Anti-BMP-2 Antibody Mouse Monoclonal WB,ELISA,IP 10426-MM14
    Anti-BMP-2 Antibody Mouse Monoclonal ICC/IF,IF 10426-MM22
    Anti-BMP-2 Antibody Mouse Monoclonal ELISA(Cap) 10426-MM23
    Anti-BMP-2 Antibody Rabbit Polyclonal ELISA 10426-T16
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