The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|人 BPHL 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG15763-ACG|
|人 BPHL 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG15763-ACR|
|人 BPHL 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG15763-CF|
|人 BPHL 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG15763-CH|
|人 BPHL 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG15763-CM|
|人 BPHL 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG15763-CY|
|人 BPHL 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG15763-NF|
|人 BPHL 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG15763-NH|
|人 BPHL 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG15763-NM|
|人 BPHL 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG15763-NY|
|人 BPHL 基因ORF全长cDNA克隆(表达载体)||HG15763-UT|
BPHL is a member of the serine protease family. BPHL is expressed large quantities in liver and kidney and in minor quantities in heart, intestine and skeletal muscle. BPHL is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol. It catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. BPHL also activates valacyclovir to acyclovir. It may play a role in detoxification processes.