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人 BTN3A1 / CD277 基因ORF全长cDNA(克隆载体)

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     BTN3A1 cDNA Clone产品信息
    NCBI注册码:BC121800
    参考序列ORF长度:1542bp
    cDNA基因描述:Full length Clone DNA of Homo sapiens butyrophilin, subfamily 3, member A1.
    分子别称:BTF5, BT3.1, CD277
    分子种属:Human
    载体:pGEM-T Vector
    质粒:pGEM-BTN3A1
    限制性酶切位点:
    Tag序列:
    序列信息:Identical with the Gene Bank Ref. ID sequence.
    测序引物:SP6 and T7 or M13-47 and RV-M
    ( We provide with BTN3A1 qPCR primers for gene expression analysis, HP104560 )
    启动子:
    Application:
    抗生素(大肠杆菌):Ampicillin
    抗生素(哺乳动物细胞):
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    pGEM-T Vector Information

    The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

    pGEM-T Simple Usage Suggestion:

    The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

    Vector Sequence Download
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    研究背景

    BTN3A1 has the structure of a type I receptor of the Ig superfamily and is part of a family of seven BTN receptors encoded by genes in the MHC. BTN molecules are composed of two Ig domains (IgV, IgC2), a single transmembrane domain, and a large carboxyl-terminal domain termed B30.2 (or PRYSPRY) located in the cell cytoplasm. There are three human BTN3A loci, BTN3A1, BTN3A2, and BTN3A3, and clear orthologs of BTN3A molecules, now called CD277, are absent from the mouse genome. Despite its similarity to B7 molecules, BTN3A1 was proposed to act not as a coreceptor or costimulatory molecule, but rather to directly present pAg to the γδ TCR in a manner analogous to MHC-restricted peptide presentation. However, this model of BTN3A1 function has been challenged by conflicting data, which show pAg binding to a positively charged pocket in the cytosolic B30.2 domain, and that BTN3A1 does not directly engage the γδ TCR. This contradictory picture has emerged as a result of the complexity of the system and in particular by the use of endogenous and exogenous routes of Ag delivery in in vitro assays.

    Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

    参考资料
  • Rhodes DA, Chen H-C, Price AJ, et al. Activation of human γδ T cells by cytosolic interactions of BTN3A1 with soluble phosphoantigens and the cytoskeletal adaptor periplakin. Journal of immunology (Baltimore, Md : 1950). 2015;194(5):2390-2398.
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    货号: HG15973-G
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    Datasheet & Documentation

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