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人 BPIFB1 / LPLUNC1 基因ORF全长cDNA(克隆载体)

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     C20ORF114 cDNA Clone产品信息
    NCBI注册码:BC008429
    参考序列ORF长度:1455bp
    cDNA基因描述:Full length Clone DNA of Homo sapiens chromosome 20 open reading frame 114.
    分子别称:LPLUNC1, C20orf114, BPIFB1
    分子种属:Human
    载体:pGEM-T Vector
    质粒:pGEM-C20ORF114
    限制性酶切位点:
    Tag序列:
    序列信息:Identical with the Gene Bank Ref. ID sequence.
    测序引物:SP6 and T7 or M13-47 and RV-M
    ( We provide with BPIFB1 qPCR primers for gene expression analysis, HP101984 )
    启动子:
    Application:
    抗生素(大肠杆菌):Ampicillin
    抗生素(哺乳动物细胞):
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    pGEM-T Vector Information

    The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

    pGEM-T Simple Usage Suggestion:

    The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

    Vector Sequence Download
    Product nameProduct name
    研究背景

    BPIFB1, also known as LPLUNC1, belongs to the BPI/LBP/Plunc superfamily, plunc family. BPIFB1 may be involved in the innate immune response to bacterial exposure in the mouth, nasal cavities, and lungs. BPIFB1 is expressed in the upper respiratory tract and oral cavity, which may function in host defence. The expression of BPIF proteins is associated with CF lung disease in humans and mice. It is unclear if this elevation of protein production, which results from phenotypic alteration of the cells within the diseased epithelium, plays a role in the pathogenesis of the disease. BPIFB1 is an abundant, secreted product of goblet cells and minor mucosal glands of the respiratory tract and oral cavity and suggest that the protein functions in the complex milieu that protects the mucosal surfaces in these locations.

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    货号: HG13275-G
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