Anti-LAMP3 Antibody (Rabbit Monoclonal antibody) General Information
Reacts with: Human
Recombinant Human CD208 / DC-LAMP / LAMP3 protein (Catalog#10527-H08H)
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD208 / DC-LAMP / LAMP3 (rh CD208 / DC-LAMP / LAMP3; Catalog#10527-H08H; NP_055213.2; Lys28-Thr381).
Monoclonal Rabbit IgG Clone #007
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-LAMP3 Antibody (Rabbit Monoclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-LAMP3 Antibody (Rabbit Monoclonal antibody) Images
Immunofluorescence staining of Human LAMP3 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human LAMP3 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and countstained with DAPI (blue). Positive staining was localized to cytoplasm.
Anti-LAMP3 Antibody Alternative Names
Anti-CD208 Antibody;Anti-DC LAMP Antibody;Anti-DC-LAMP Antibody;Anti-DCLAMP Antibody;Anti-LAMP Antibody;Anti-LAMP-3 Antibody;Anti-TSC403 Antibody
LAMP3 Background Information
Dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208, also known as LAMP3, is a member of the lysosomal associated membrane protein (LAMP) family, which is specifically expressed by human dendritic cells (DCs) upon activation and therefore serves as marker of human DC maturation. Confocal and immunoelectron microscopy showed that mouse DC-LAMP protein co-localizes with lbm180, a specific marker for the limiting membrane of lamellar bodies that contain surfactant protein B. The present study demonstrates that DC-LAMP is constitutively expressed by mouse, sheep, and human type II pneumocytes. DC-LAMP is constitutively expressed in normal type II pneumocytes. DC-LAMP is detected first in activated human DC within MHC class II molecules-containing compartments just before the translocation of MHC class II-peptide complexes to the cell surface, suggesting a possible involvement in this process. Furthermore, overexpression of LAMP3 is actively involved in tumor invasion through increased migration into lymph-vascular spaces.
lysosomal associated membrane protein 3
Salaun B, et al. (2003) Cloning and characterization of the mouse homologue of the human dendritic cell maturation marker CD208/DC-LAMP. Eur J Immunol. 33(9): 2619-29.Salaun B, et al. (2004) CD208/dendritic cell-lysosomal associated membrane protein is a marker of normal and transformed type II pneumocytes. Am J Pathol. 164(3): 861-71.Ishigami S, et al. (2010) Prognostic value of CD208-positive cell infiltration in gastric cancer. Cancer Immunol Immunother. 59(3): 389-95.