Anti-LAMP3 Antibody

Cat: 10527-R007

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Anti-LAMP3 Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-LAMP3 Antibody
Validated applications
ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human LAMP3
Immunogen
Recombinant Human CD208 / DC-LAMP / LAMP3 protein (Catalog#10527-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD208 / DC-LAMP / LAMP3 (rh CD208 / DC-LAMP / LAMP3; Catalog#10527-H08H; NP_055213.2; Lys28-Thr381).
Source
Monoclonal Rabbit IgG Clone #007
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-LAMP3 Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution Notes
ICC-IF 1:20-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-LAMP3 Antibody (Rabbit Monoclonal antibody) Images

Human CD208/DC-LAMP Immunofluorescence(IF) 2896
Immunofluorescence staining of Human LAMP3 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human LAMP3 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and countstained with DAPI (blue). Positive staining was localized to cytoplasm.

Anti-LAMP3 Antibody Alternative Names

Anti-CD208 Antibody;Anti-DC LAMP Antibody;Anti-DC-LAMP Antibody;Anti-DCLAMP Antibody;Anti-LAMP Antibody;Anti-LAMP-3 Antibody;Anti-TSC403 Antibody

LAMP3 Background Information

Dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208, also known as LAMP3, is a member of the lysosomal associated membrane protein (LAMP) family, which is specifically expressed by human dendritic cells (DCs) upon activation and therefore serves as marker of human DC maturation. Confocal and immunoelectron microscopy showed that mouse DC-LAMP protein co-localizes with lbm180, a specific marker for the limiting membrane of lamellar bodies that contain surfactant protein B. The present study demonstrates that DC-LAMP is constitutively expressed by mouse, sheep, and human type II pneumocytes. DC-LAMP is constitutively expressed in normal type II pneumocytes. DC-LAMP is detected first in activated human DC within MHC class II molecules-containing compartments just before the translocation of MHC class II-peptide complexes to the cell surface, suggesting a possible involvement in this process. Furthermore, overexpression of LAMP3 is actively involved in tumor invasion through increased migration into lymph-vascular spaces.

Full Name
lysosomal associated membrane protein 3
References
  • Salaun B, et al. (2003) Cloning and characterization of the mouse homologue of the human dendritic cell maturation marker CD208/DC-LAMP. Eur J Immunol. 33(9): 2619-29.
  • Salaun B, et al. (2004) CD208/dendritic cell-lysosomal associated membrane protein is a marker of normal and transformed type II pneumocytes. Am J Pathol. 164(3): 861-71.
  • Ishigami S, et al. (2010) Prognostic value of CD208-positive cell infiltration in gastric cancer. Cancer Immunol Immunother. 59(3): 389-95.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-LAMP3 Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 10527-T60
    Anti-LAMP3 Antibody Rabbit Polyclonal ELISA 10527-RP01
    Anti-LAMP3 Antibody Rabbit Monoclonal ELISA(Cap) 10527-R020
    Anti-LAMP3 Antibody Rabbit Polyclonal ELISA 10527-T16
    Anti-LAMP3 Antibody Mouse Monoclonal ELISA 10527-MM02
    Anti-LAMP3 Antibody Rabbit Monoclonal ELISA,ELISA(Det) 10527-R002
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