All CD33 reagents are produced in house and quality controlled, including 19 CD33 Antibody, 39 CD33 Gene, 8 CD33 Lysate, 8 CD33 Protein, 2 CD33 qPCR. All CD33 reagents are ready to use.
Recombinant CD33 proteins are expressed by HEK293 Cells with fusion tags as C-cleavage, C-His, C-mouse IgG1-Fc, C-human IgG1-Fc.
CD33antibodies are validated with different applications, which are FCM, ELISA, WB, IP.
CD33cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each CD33 of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
Myeloid cell surface antigen CD33 also known as Sialic acid binding Ig-like lectin 3, CD33 antigen or Siglec-3, is a member of the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. This Single-pass type I membrane protein contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain. CD33 /Siglec-3 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. CD33 /Siglec-3 preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33/Siglec-3 induces apoptosis in acute myeloid leukemia (in vitro). CD33/Siglec-3 can function as a sialic acid-dependent cell adhesion molecule and that binding can be modulated by endogenous sialoglycoconjugates when CD33 is expressed in a plasma membrane.