Anti-CD34 Antibody

Price:
Size:
Number:

Anti-CD34 Antibody (Rabbit Monoclonal 抗体) 产品信息

产品名称
Anti-CD34 Antibody
经验证的应用
WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IF,IP
交叉反应
Reacts with: Mouse
特异性
Mouse CD34
免疫原
Recombinant Mouse CD34 Protein (Catalog#50589-M08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD34 (rM CD34; Catalog#50589-M08H; NP_001104529.1; Met 1-Thr 287).
来源
Monoclonal Rabbit IgG Clone #013
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD34 Antibody (Rabbit Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
ELISA 1:25000-1:50000
IHC-P 1:2000-1:10000
ICC-IF 1:100-1:500
FCM 1:100-1:500
IP 0.2-1 μL/mg of lysate
ELISA(Det) 1:5000-1:50000
Notes
ELISA(Det): This antibody will detect Mouse CD34 in ELISA pair set (Catalog: # SEK50589). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 50589-R028).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD34 Antibody (Rabbit Monoclonal 抗体) 图片

Immunochemical Staining of mouse CD34 in mouse stomach with Rabbit Monoclonal Antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to vascular endothelial cells.
Immunochemical Staining of mouse CD34 in mouse kidney with Rabbit Monoclonal Antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to vascular endothelial cells.

Anti-CD34 rabbit monoclonal antibody at 1:500 dilution

Lane A: TF-1 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:41 kDa

Observed band size:130 kDa

(We are unsure as to the identity of these extra bands.)

Flow cytometric analysis of CD34 expression on M1 cells. Cells were stained with purified anti-Mouse CD34, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Mouse CD34 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

0.5 µL anti-Mouse CD34 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse CD34 rabbit monoclonal antibody,at 1:350 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 41 kDa

Observed band size: 130 kDa

Immunofluorescence staining of CD34 in M1 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD34 monoclonal antibody (1:300) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).

Anti-CD34 Antibody: Alternative Names

Anti-AU040960 Antibody

CD34 Background Information

Cluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias.
Full Name
CD34 molecule
References
  • Hogan CJ, et al. (2002) Differential long-term and multilineage engraftment potential from subfractions of human CD34+ cord blood cells transplanted into NOD/SCID mice. Proc Nat Acad Sci USA. 99 (1): 413-8.
  • Nielsen JS,et al. (2009) CD34 is a key regulator of hematopoietic stem cell trafficking to bone marrow and mast cell progenitor trafficking in the periphery. Microcirculation. 16(6): 487-96.
  • Mastrandrea F,et al. (2009) CD34+ hemopoietic precursor and stem cells traffic in peripheral blood of celiac patients is significantly increased but not directly related to epithelial damage severity. Eur Ann Allergy Clin Immunol. 40(3): 90-103.
  • Pasquet S,et al. (2009) Long-term benefit of intracardiac delivery of autologous granulocyte-colony-stimulating factor-mobilized blood CD34+ cells containing cardiac progenitors on regional heart structure and function after myocardial infarct. Cytotherapy. 11(8): 1002-15.
添加购物车成功! 添加购物车失败!