|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 CEACAM5/CEA/CD66e 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG11077-ACG|
|人 CEACAM5/CEA/CD66e 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG11077-ACR|
|人 CEACAM5/CEA/CD66e 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG11077-CF|
|人 CEACAM5/CEA/CD66e 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG11077-CH|
|人 CEACAM5/CEA/CD66e 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG11077-CM|
|人 CEACAM5/CEA/CD66e 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG11077-CY|
|人 CEACAM5/CEA/CD66e 基因ORF全长cDNA(克隆载体)||HG11077-M|
|人 CEACAM5/CEA/CD66e 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG11077-NF|
|人 CEACAM5/CEA/CD66e 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG11077-NH|
|人 CEACAM5/CEA/CD66e 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG11077-NM|
|人 CEACAM5/CEA/CD66e 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG11077-NY|
|人 CEACAM5/CEA/CD66e 基因ORF全长cDNA克隆(表达载体)||HG11077-UT|
CEACAM5, also known as CEA or D66e, belongs to the large CEACAM subfamily of immunoglobulin superfamily. CEACAM5 is expressed primarily by epithelial cells, and is synthesized as a glycoprotein with a MW of 180 kDa comprising 60% carbohydrate. CEACAM5 contains one Ig-like V-type domain at the N-terminus, followed by six Ig-like C2-type domain and a GPI anchor, and exists as a homodimer. CEACAM5 and CEACAM6 are overexpressed in many cancers and are associated with adhesion and invasion. CEACAM5 can mediate cell-cell adhesion through homotypic and heterotypic interactions. It functions as a homotypic intercellular adhesion molecule and serves as a widely used tumor marker, since it is expressed at higher levels in tumorous tissues than in corresponding normal tissues. CEACAM5 has also been shown to contribute to tumorigenicity by inhibiting cellular differentiation. In addition, CEACAM5 is identified as the host receptor for the Dr family of adhesins of E.Coli, and the binding of E.coli Dr adhesins leads to dissociation of the CEACAM5 homodimer.