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CD68 抗体, 兔多抗, 抗原亲和纯化

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Human CD68 抗体产品信息
免疫原:Recombinant Human CD68 protein (Catalog#11192-H08B1)
克隆号:
Ig 类型:Rabbit IgG
浓度:
内毒素:
缓冲液:0.2 μm filtered solution in PBS with 5% trehalose
抗体制备方法:Produced in rabbits immunized with purified, recombinant Human CD68 (rh CD68; Catalog#11192-H08B1; P34810-1; Met1-Ser319). CD68 specific IgG was purified by Human CD68 affinity chromatography.
Human CD68 抗体使用指导
特异性:Human CD68
推荐应用:ELISA, IHC-P

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD68. The detection limit for Human CD68 is approximately 0.00245 ng/well.

IHC-P: 0.05-2 μg/mL

储存方法:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CD68 抗体IHC实验图片
CD68 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Caption:
Immunochemical staining of human CD68 in human liver (cirrhosis) with rabbit polyclonal antibody (0.1 µg/mL, formalin-fixed paraffin embedded sections).
CD68 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[点击查看大图]
Caption:
Immunochemical staining of human CD68 in human lung with rabbit polyclonal antibody (0.1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to macrophages.
Other CD68 抗体 Products
CD68 研究背景

Macrosialin, also known as CD68 and Gp110, is a single-pass type I  membrane protein which belongs to the LAMP family. CD68 is highly expressed by blood monocytes and tissue macrophages. It is also expressed in lymphocytes, fibroblasts and endothelial cells. CD68 is expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. CD68 plays a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It is a commonly used marker for macrophages. However, a number of studies have shown that CD68 antibodies react with other hematopoietic and non-hematopoietic cell types, suggesting that CD68 may not be a macrophage-specific antigen. CD68 binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

Human CD68 参考资料
  • Strobl H. et al., 1995, Br J Haematol. 90 (4): 774-82.
  • Ogawa Y. et al., 1995, Pathol Int. 45 (9): 698-701.
  • Sadovnikova E. et al., 2002,Leukemia. 16 (10): 2019-26.
  • Gottfried E. et al., 2008, Scand J Immunol. 67 (5): 453-63.
  • Strojnik T. et al., 2009, Anticancer Res. 29 (8): 3269-79.
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    货号: 11192-RP02-50
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