Anti-CD73 Antibody

1/1
Price:
Size:
Number:

Anti-CD73 Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-CD73 Antibody
经验证的应用
WB,ELISA
交叉反应
Reacts with: Human
特异性
Human CD73
免疫原
Recombinant Human CD73 / NT5E protein (Catalog#10904-H08H)
制备方法
Produced in rabbits immunized with purified, recombinant Human CD73 / NT5E (rh CD73 / NT5E; Catalog#10904-H08H; NP_002517.1; Met 1-Lys 547). CD73 / NT5E specific IgG was purified by Human CD73 / NT5E affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD73 Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
ELISA 1:25000-1:50000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD73 Antibody (Rabbit Polyclonal 抗体) 图片

Anti-NT5E rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Membrane Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:63 kDa

Observed band size:63 kDa

Anti-CD73 Antibody: 别称

Anti-CALJA Antibody; Anti-CD73 Antibody; Anti-E5NT Antibody; Anti-eN Antibody; Anti-eNT Antibody; Anti-NT Antibody; Anti-NT5 Antibody; Anti-NTE Antibody

CD73 背景信息

5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.
全称
5'-nucleotidase, ecto (CD73)
参考文献
  • Resta R. et al., 1997, Cell Signal. 9 (2): 131-9.
  • Yamashita Y. et al., 1998, Eur J Immunol. 28 (10): 2981-90.
  • Louis NA. et al., 2008, J Immunol. 180 (6): 4246-55.
  • Grünewald JK. et al., 2010, J Inflamm. 7 (1): 10.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后