Anti-CD74 Antibody

Cat: 11091-MM01

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All CD74 Reagents

Anti-CD74 Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-CD74 Antibody
Validated applications
IHC-P,FCM
Species reactivity
Reacts with: Human
Specificity
Human CD74
Immunogen
Recombinant Human CD74 protein (Catalog#11091-H07H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD74 (rh CD74; Catalog#11091-H07H; NP_005219; Met668-Ala1210). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #01
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-CD74 Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution Notes
IHC-P 1:500-1:2000  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD74 Antibody (Mouse Monoclonal antibody) Images

Human CD74 Immunohistochemistry(IHC) 4469
Immunochemical staining of human CD74 in human tonsil with mouse monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Human CD74 Immunohistochemistry(IHC) 4475
Immunochemical staining of human CD74 in human colon carcinoma with mouse monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Human CD74 Immunohistochemistry(IHC) 4476
Immunochemical staining of human CD74 in human appendix with mouse monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Human CD74 Flow Cytometry (FC) 5233
Flow cytometric analysis of Human CD74 expression on human whole blood lymphocytes. Cells were stained with purified anti-Human CD74, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.

Anti-CD74 Antibody Alternative Names

Anti-DHLAG Antibody;Anti-HLADG Antibody;Anti-Ia-GAMMA Antibody;Anti-II Antibody

CD74 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD74, also known as HLA class2 histocompatibility antigen gamma chain and HLA-DR antigens-associated invariant chain, is a polypeptide involved in the formation and transport of MHC class2 protein. CD74 is expressed by B cells, macrophages, and Reed-Sternberg cells. When MHC class 2 protein was in the rough ER, its peptide-binding cleft was blocked by CD74 to prevent it from interacting with the endogenous peptides. CD74 also serves to facilitate MHC class2's export from ER.

Full Name
CD74 molecule, major histocompatibility complex, class II invariant chain
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Moynes R, et al. (1997) A marker to distinguish atypical fibroxanthoma from malignant fibrous histiocytoma. Cancer. 79 (11): 2115-24.
  • Gabrielle FA, et al. (2008) Regulation of dendritic cell migration by CD74, the MHC class 2-associated invariant chain. Science. 322 (5908): 1705-10.
  • Leng L, et al. (2003) MIF signal transduction initiated by binding to CD74. JEM.197 (11): 1467-76.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD74 Antibody Rabbit Polyclonal WB 102339-T32
    Anti-CD74 Antibody (APC) Mouse Monoclonal FCM 11091-M101-A
    Anti-CD74 Antibody (PE) Mouse Monoclonal FCM 11091-MM01-P
    Anti-CD74 Antibody Rabbit Polyclonal ELISA 11091-RP01
    Anti-CD74 Antibody (PE) Mouse Monoclonal FCM 11091-M101-P
    Anti-CD74 Antibody Rabbit Polyclonal ELISA 11091-T16
    Anti-CD74 Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 11091-RP02
    Anti-CD74 Antibody (APC) Mouse Monoclonal FCM 11091-MM01-A
    Anti-CD74 Antibody Rabbit Monoclonal ELISA,IHC-P 11091-R301
    Anti-CD74 Antibody Mouse Monoclonal FCM 11091-M101
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