Anti-CD8 alpha Antibody

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Anti-CD8 alpha Antibody (Rabbit Monoclonal 抗体) 产品信息

产品名称
Anti-CD8 alpha Antibody
经验证的应用
FCM,ICC/IF,IF
交叉反应
Reacts with: Mouse
特异性
Mouse CD8 alpha
免疫原
Recombinant Mouse CD8a / Lyt2 protein (Catalog#50389-M08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD8a / Lyt2 (rM CD8a / Lyt2; Catalog#50389-M08H; NP_001074579.1; Met1-Tyr196).
来源
Monoclonal Rabbit IgG Clone #208
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD8 alpha Antibody (Rabbit Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
ICC-IF 1:20-1:100
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD8 alpha Antibody (Rabbit Monoclonal 抗体) 图片

Immunofluorescence staining of Mouse CD8a in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD8a monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.
Flow cytometric analysis of Mouse CD8α expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse CD8α, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Anti-CD8 alpha Antibody: 别称

Anti-BB154331 Antibody; Anti-Ly-2 Antibody; Anti-Ly-35 Antibody; Anti-Ly-B Antibody; Anti-Lyt-2 Antibody

CD8 alpha 背景信息

T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
全称
CD8a molecule
参考文献
    References Devine, L. et al., 2000, J Immunol. 164 (2): 833-8. Arcaro, A. et al., 2000, J Immunol. 165 (4): 2068-76. Saha, K. et al., 2001, Nat Med. 7 (1): 65-72. Romero, P. et al., 2005, Eur J Immunol. 35 (11): 3092-4.
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