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人 CDC27 基因ORF全长cDNA(克隆载体)

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     CDC27 cDNA Clone产品信息
    NCBI注册码:BC011656
    参考序列ORF长度:2493bp
    cDNA基因描述:Full length Clone DNA of Homo sapiens cell division cycle 27.
    分子别称:APC3, HNUC, NUC2, ANAPC3, CDC27Hs, D0S1430E, D17S978E
    分子种属:Human
    载体:pGEM-T Vector
    质粒:pGEM-CDC27
    限制性酶切位点:
    Tag序列:
    序列信息:Identical with the Gene Bank Ref. ID sequence.
    测序引物:SP6 and T7 or M13-47 and RV-M
    ( We provide with CDC27 qPCR primers for gene expression analysis, HP104557 )
    启动子:
    Application:
    抗生素(大肠杆菌):Ampicillin
    抗生素(哺乳动物细胞):
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    pGEM-T Vector Information

    The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

    pGEM-T Simple Usage Suggestion:

    The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

    Vector Sequence Download
    Product nameProduct name
    研究背景

    Cdc27, a component of the anaphase-promoting complex (APC/C), as a novel binding partner of MCPH1. APC/C is an E3 ubiquitin ligase that controls the cell cycle. A combination of in vitro and in vivo experiments revealed that the interaction between MCPH1 and Cdc27 is dependent on the phosphorylation of Cdc27 and is mediated by the C-terminal BRCT domains of MCPH1.

    Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

    参考资料
  • Singh N, Wiltshire TD, Thompson JR, Mer G, Couch FJ. Molecular Basis for the Association of Microcephalin (MCPH1) Protein with the Cell Division Cycle Protein 27 (Cdc27) Subunit of the Anaphase-promoting Complex. The Journal of Biological Chemistry. 2012;287(4):2854-2862.
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    货号: HG15970-G
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