( We provide with CDNF qPCR primers for gene expression analysis, HP104288 )
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|人 CDNF/ARMETL1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG15691-ACG|
|人 CDNF/ARMETL1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG15691-ACR|
|人 CDNF/ARMETL1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG15691-CF|
|人 CDNF/ARMETL1 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG15691-CH|
|人 CDNF/ARMETL1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG15691-CM|
|人 CDNF/ARMETL1 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG15691-CY|
|人 CDNF/ARMETL1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG15691-NF|
|人 CDNF/ARMETL1 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG15691-NH|
|人 CDNF/ARMETL1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG15691-NM|
|人 CDNF/ARMETL1 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG15691-NY|
|人 CDNF/ARMETL1 基因ORF全长cDNA克隆(表达载体)||HG15691-UT|
Cerebral Dopamine Neurotrophic Factor (CDNF), also known as ARMETL1 (ARMET-like protein 1), is a secreted protein with eight conserved cysteine residues, predicting a unique protein fold and defining a new, evolutionarily conserved protein family. CDNF is a novel neurotrophic factor with strong trophic activity on dopaminergic neurons comparable to that of glial cell line-derived neurotrophic factor (GDNF). CDNF/ARMETL1 is a evolutionary conserved protein which can protect and restore the function of dopaminergic neurons in the rat model of Parkinson's disease, suggesting that CDNF might be beneficial for the treatment of Parkinson's disease. CDNF is widely expressed in neurons in several brain regions including cerebral cortex, hippocampus, substantia nigra, striatum and cerebellum. Human CDNF is glycosylated and secreted from transiently transfected cells. CDNF promotes the survival, growth, and function of dopamine-specific neurons and is expressed in brain regions that undergo cocaine-induced neuroplasticity.