CNTF Protein, Human, Recombinant (His Tag)

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CNTF Protein, Human, Recombinant (His Tag): 产品信息

纯度
> 90 % as determined by SDS-PAGE
内毒素
Please contact us for more information.
生物活性
1. Measured by its binding ability in a functional ELISA. Immobilized human His-CNTF (cat:11841-H07E) at 10 μg/ml (100 μl/well) can bind biotinylated rat CNTFR-His (Cat:80019-R08B). The EC50 of biotinylated rat CNTFR-His (Cat:80019-R08B) is 60-140 ng/ml.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 1-4 μg/mL.
蛋白构建
A DNA sequence encoding the human CNTF (NP_000605.1) (Ala 2-Met 200) was expressed, with a polyhistide tag at the N-terminus.
NP No.
表达宿主
E. coli
种属
Human
预测 N 端
Met
分子量
The recombinant human CNTF consisting of 214 amino acids and has a calculated molecular mass of 24.5 kDa. The apparent molecular mass of the protein is approximately 27-30 kDa in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CNTF Protein, Human, Recombinant (His Tag): 图片

CNTF Protein, Human, Recombinant (His Tag): 别称

HCNTF Protein, Human

CNTF 背景信息

Ciliary neurotrophic factor (CNTF) is a member of the cytokine family. It is a polypeptide hormone that have functions in promoting neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. It's actions appear to be restricted to the nervous system. Ciliary neurotrophic factor (CNTF) has biological effects through the activation of a multi- subunit receptor complex, consisting of an extracelluar CNTF binding subunit (CNTFα) and two transmembrane signal transduction proteins: glycoprotein gp13 and LIF receptor. CNTF is considered as a potent survival factor of neurons and oligodendrocyteands may be relevant in reducing tissue destruction during inflammatory attacks. CNTF also is a survival factor for neurons of the peripheral sensory sympathetic and ciliary ganglia. It has been reported that CNTF could be an agent that has therapeutic potential and possibly induces differentiation of large multipolar ganglionic phenotype in a subset of progenitors.
全称
ciliary neurotrophic factor
参考文献
  • Dutt K, et al. (2010) Ciliary neurotrophic factor: a survival and differentiation inducer in human retinal progenitors. In Vitro Cell Dev Biol Anim. 46 (7) : 635-46.
  • Lam A, et al.(1991) Sequence and structural organization of the human gene encoding ciliary neurotrophic factor. Gene 102 (2) : 271–6.
  • Bazan JF. ( 1991) Neuropoietic cytokines in the hematopoietic fold. Neuron 7 (2) : 197–208.
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    CNTF related areas, pathways, and other information

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