快速订购

小鼠 Contactin 5/CNTN5 基因ORF全长cDNA(克隆载体)

产品数据评论相关产品实验方法
小鼠 CNTN5 cDNA Clone产品信息
NCBI注册码:NM_001033359.2
参考序列ORF长度:2682bp
cDNA基因描述:Full length Clone DNA of Mus musculus contactin 5.
分子别称:NB-2, Gm507, 6720426O10Rik, A830025P08Rik, Cntn5
分子种属:Mouse
载体:pGEM-T Vector
质粒:pGEM-mCNTN5
限制性酶切位点:
Tag序列:
序列信息:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1072 G/A resulting in the amino acid Ala substitution by Thr and 948 G/T not causing the amino acid variation.
测序引物:SP6 and T7 or M13-47 and RV-M
( We provide with CNTN5 qPCR primers for gene expression analysis, MP201049 )
启动子:
Application:
抗生素(大肠杆菌):Ampicillin
抗生素(哺乳动物细胞):
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Product nameProduct name
研究背景

Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed mainly in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2(TAG-1), Contactin-3(BIG-1), BIG-2, Contactin-5(NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-5, also known as NB-2, is one of the neural recognition molecules in the contactin subgroup. Contactin-5 is expressed in brain and kidney and at very low level in placenta. In brain, Contactin-5 is highly expressed in the occipital lobe, amygdala, cerebral cortex, frontal lobe, thalamus and temporal lobe. Mice deficient in the Contactin-5 gene exhibit aberrant responses to acoustic stimuli. Contactin-5 may play a role in maturation of glutamatergic synapses in the brainstem during the final stages of auditory development. Contactin-5 gene may contribute to human neurological disorders.

参考资料
  • Kamei Y, et al. (2000) Human NB-2 of the contactin subgroup molecules: chromosomal localization of the gene (CNTN5) and distinct expression pattern from other subgroup members. Genomics 69(1):113-9.
  • Ogawa J, et al. (2001) Neural recognition molecule NB-2 of the contactin/F3 subgroup in rat: Specificity in neurite outgrowth-promoting activity and restricted expression in the brain regions. J Neurosci Res. 65(2):100-10.
  • Li H, et al. (2003) Aberrant responses to acoustic stimuli in mice deficient for neural recognition molecule NB-2. Eur J Neurosci. 17(5):929-36.
  • Shimoda Y, et al. (2009) Contactins: Emerging key roles in the development and function of the nervous system. Cell adhesion & migration 3(1):64-70.
  • Toyoshima M, et al. (2009) Preferential localization of neural cell recognition molecule NB-2 in developing glutamatergic neurons in the rat auditory brainstem. J Comp Neurol. 513(4):349-62.
  • Size / Price
    货号: MG51092-G
    目录价: 
    单价:      (You Save: )
    规格:
    数量:+-
    库存In Stock
    加入购物车大包装查询
    Contact Us
    产品图片
        您曾浏览过的产品
          产品数据可能随批次变化,最新数据请详见随货说明书。
          请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”