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人 COL21A1 Gene cDNA Clone (full-length ORF Clone)

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     COL21A1 cDNA Clone产品信息
    NCBI注册码:NM_030820.3
    参考序列ORF长度:2874
    cDNA基因描述:ORF Clone of Homo sapiens collagen, type XXI, alpha 1 DNA.
    分子别称:FP633, COLA1L, dJ708F5.1, dJ682J15.1, COL21A1
    分子种属:Human
    载体:pGEM-T Vector
    质粒:
    限制性酶切位点:
    Tag序列:
    序列信息:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1355 G/A resulting in the amino acid Gly substitution by Glu and 90 T/C,438 A/G not causing the amino acid variation.
    测序引物:
    启动子:
    Application:
    抗生素(大肠杆菌):Ampicillin
    抗生素(哺乳动物细胞):
    运输方式:
    储存方法:The lyophilized plasmid can be stored at ambient temperature for three months.
    pGEM-T Vector Information

    The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

    pGEM-T Simple Usage Suggestion:

    The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

    Vector Sequence Download
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