( We provide with COQ7 qPCR primers for gene expression analysis, RP300682 )
pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|大鼠 COQ7 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||RG80718-ACG|
|大鼠 COQ7 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||RG80718-ACR|
|大鼠 COQ7 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||RG80718-ANG|
|大鼠 COQ7 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||RG80718-ANR|
|大鼠 COQ7 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||RG80718-CF|
|大鼠 COQ7 基因ORF全长cDNA克隆(表达载体), C-His 标签||RG80718-CH|
|大鼠 COQ7 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||RG80718-CM|
|大鼠 COQ7 基因ORF全长cDNA克隆(表达载体), C-HA 标签||RG80718-CY|
|大鼠 COQ7 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||RG80718-NF|
|大鼠 COQ7 基因ORF全长cDNA克隆(表达载体), N-His 标签||RG80718-NH|
|大鼠 COQ7 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||RG80718-NM|
|大鼠 COQ7 基因ORF全长cDNA克隆(表达载体), N-HA 标签||RG80718-NY|
|大鼠 COQ7 基因ORF全长cDNA(克隆载体)||RG80718-U|
|大鼠 COQ7 基因ORF全长cDNA克隆(表达载体)||RG80718-UT|
Ubiquinone biosynthesis protein COQ7 homolog, also known as Coenzyme Q biosynthesis protein 7 homolog, Timing protein clk-1 homolog and COQ7, is a mitochondrion inner membrane and peripheral membrane protein which belongs to the COQ7 family. It is expressed dominantly in heart and skeletal muscle. COQ7 is synthesized as a preprotein that is imported into the mitochondrial matrix, where the sequence is cleaved off and the mature protein becomes loosely associated with the inner membrane. This enzyme is responsible for the hydroxylation of 5-demethoxyubiquinone to 5-hydroxyubiquinone. Human COQ7 protein is mostly helical, and contains an alpha-helical membrane insertion. It has a potential N-glycosylation site, a phosphorylation site for protein kinase C and another for casein kinase II, and three N-myristoylation sites. COQ7 is involved in lifespan determination in ubiquinone-independent manner. It is also involved in ubiquinone biosynthesis. COQ7 is potential central metabolic regulator.