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人 IL8Rb/CXCR2 基因ORF全长cDNA克隆(表达载体), C-His 标签

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Human IL8Rb/CXCR2 cDNA Clone产品信息
NCBI注册码:NM_001557.2
参考序列ORF长度:1083bp
cDNA基因描述:Full length Clone DNA of interleukin 8 receptor, beta with His tag.
分子别称:CD182, CXCR2, IL8R2, IL8RA, CMKAR2, CDw128b
分子种属:Human
载体:pCMV/hygro-His
质粒:pCMV-CXCR2-10his
限制性酶切位点:KpnI + XhoI (5.5kb + 1.11kb)
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列信息:Identical with the Gene Bank Ref.ID sequence.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
Human IL8Rb/CXCR2 基因质粒图
Human CXCR2 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged
pCMV/hygro-HIs Vector Information
 
Vector Name pCMV/hygro-His
Vector Size 5687bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive ,Stable / Transient
Promoter CMV
Antibiotic Resistance Ampicillin
Selection In Mammalian Cells Hygromycin
Protein Tag His
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

Schematic of pCMV/hygro-His Multiple Cloning Sites

His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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  • Human CXCR2 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged
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