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人 CYB5R1 基因ORF全长cDNA(克隆载体)

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Human CYB5R1 cDNA Clone产品信息
NCBI注册码:BC018732
参考序列ORF长度:918bp
cDNA基因描述:Full length Clone DNA of Homo sapiens cytochrome b5 reductase 1.
分子别称:B5R1, B5R.1, NQO3A2, humb5R2, CYB5R1
分子种属:Human
载体:pGEM-T Vector
质粒:pGEM-CYB5R1
限制性酶切位点:
Tag序列:
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:SP6 and T7 or M13-47 and RV-M
启动子:
Application:
抗生素(大肠杆菌):Ampicilin
抗生素(哺乳动物细胞):
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
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参考资料
  • Troncoso C, et al. (2008) Influence of electron transport proteins on the reactions catalyzed by Fusarium fujikuroi gibberellin monooxygenases. Phytochemistry. 69(3): 672-83.
  • Qian W, et al. (1998) The influence of mutation at Glu44 and Glu56 of cytochrome b5 on the protein's stabilization and interaction between cytochrome c and cytochrome b5. Biochemistry. 37 (40): 14137-50.
  • Zhu H, et al. (2000) Identification of a cytochrome b-type NAD(P)H oxidoreductase ubiquitously expressed in human cells. Proc Natl Acad Sci. 96 (26): 14742-7.
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    货号: HG13227-G
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