快速订购

人 Cadherin-11/OB-Cadherin 基因ORF全长cDNA克隆(表达载体), C-His 标签

    产品数据评论相关产品实验方法
     CDH11 cDNA Clone产品信息
    NCBI注册码:NM_001797.2
    参考序列ORF长度:2391bp
    cDNA基因描述:Full length Clone DNA of Homo sapiens cadherin 11, type 2, OB-cadherin (osteoblast) with His tag.
    分子别称:CDH11, OB, CAD11, CDHOB, OSF-4
    分子种属:Human
    载体:pCMV/hygro-His
    质粒:pCMV-CDH11-10his
    限制性酶切位点:KpnI + XhoI (5.5kb + 2.42kb)
    Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
    序列信息:Identical with the Gene Bank Ref. ID sequence.
    测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with CDH11 qPCR primers for gene expression analysis, HP100151 )
    启动子:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    抗生素(大肠杆菌):Ampicillin
    抗生素(哺乳动物细胞):Hygromycin
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    pCMV/hygro-HIs Vector Information
     
    Vector Name pCMV/hygro-His
    Vector Size 5687bp
    Vector Type Mammalian Expression Vector
    Expression Method Constiutive ,Stable / Transient
    Promoter CMV
    Antibiotic Resistance Ampicillin
    Selection In Mammalian Cells Hygromycin
    Protein Tag His
    Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
    Reverse:BGH(TAGAAGGCACAGTCGAGG)

    Schematic of pCMV/hygro-His Multiple Cloning Sites

    His Tag Info

    A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

    Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

    Product nameProduct name
    产品数据可能随批次变化,最新数据请详见随货说明书。
    请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”