Anti-Calbindin Antibody

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Anti-Calbindin Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-Calbindin Antibody
经验证的应用
WB,IHC-P,ICC/IF,IF,IP
交叉反应
Reacts with: Human
Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
特异性
Human Calbindin
免疫原
A synthetic peptide corresponding to the N-terminus of the Human Calbindin
制备方法
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Calbindin, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Calbindin Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
IHC-P 1:500-1:2000
ICC-IF 1:300-1:10000
IP 4-8 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Calbindin Antibody (Rabbit Polyclonal 抗体) 图片

Immunofluorescence staining of CALB1 in 293 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human CALB1 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.
Immunochemical staining of human CALB1 in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

CALB1 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

4 µL anti-CALB1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-CALB1 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 30 kDa

Observed band size: 30 kDa

Anti-CALB1 rabbit polyclonal antibody at 1:500 dilution.

Lane A: Mosue kidney tissue lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:30 kDa

Observed band size:27 kDa

Anti-Calbindin Antibody: 别称

Anti-CALB Antibody; Anti-D-28K Antibody
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