BDNF cDNA ORF Clone, Canine, C-DDK (Flag®) tag

Cat: DG70029-CF
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BDNF cDNA ORF Clone, Canine, C-DDK (Flag®) tag 基本信息
基因
种属
Canine
NCBI 参考序列号
参考序列ORF长度
744 bp
描述
Full length Clone DNA of Canine brain-derived neurotrophic factor with C terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-C-FLAG
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

BDNF Background Information

BDNF is a member of the nerve growth factor family. It is highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. It also can be detected in heart, lung, skeletal muscle, testis, prostate and placenta. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. During development, BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. It participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. It functions as the major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

Full Name
brain-derived neurotrophic factor
References
  • Zigova T, et al. (1998) Intraventricular administration of BDNF increases the number of newly generated neurons in the adult olfactory bulb. Mol Cell Neurosci. 11(4):234-45.
  • Acheson A, et al. (1995) A BDNF autocrine loop in adult sensory neurons prevents cell death. Nature 374(6521):450-3.
  • Bekinschtein P, et al. (2008) BDNF is essential to promote persistence of long-term memory storage. Proc Natl Acad Sci. 105(7):2711-6.
  • BDNF cDNA ORF Clone, Canine, Related Products

    Product Name Catalog#(PDF)
    BDNF cDNA ORF Clone in Cloning Vector, Canine DG70029-G
    BDNF cDNA ORF Clone, Canine, untagged DG70029-UT
    BDNF Lentiviral cDNA ORF Clone, Canine, C-GFPSpark® tag DG70029-ACGLN
    BDNF cDNA ORF Clone, Canine, C-GFPSpark® tag DG70029-ACG
    BDNF cDNA ORF Clone, Canine, C-OFPSpark® tag DG70029-ACR
    BDNF cDNA ORF Clone, Canine, N-DDK (Flag®) tag DG70029-NF
    BDNF cDNA ORF Clone, Canine, C-His tag DG70029-CH
    BDNF cDNA ORF Clone, Canine, N-His tag DG70029-NH
    BDNF cDNA ORF Clone, Canine, C-HA tag DG70029-CY
    BDNF cDNA ORF Clone, Canine, N-HA tag DG70029-NY
    BDNF cDNA ORF Clone, Canine, C-Myc tag DG70029-CM
    BDNF cDNA ORF Clone, Canine, N-Myc tag DG70029-NM

    BDNF cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    BDNF cDNA ORF Clone in Cloning Vector, Mouse MG50240-G
    BDNF cDNA ORF Clone, Mouse, untagged MG50240-UT
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    BDNF cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50240-ACG
    BDNF cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50240-ACR
    BDNF cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50240-CF
    BDNF cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50240-NF
    BDNF cDNA ORF Clone, Mouse, C-His tag MG50240-CH
    BDNF cDNA ORF Clone, Mouse, N-His tag MG50240-NH
    BDNF cDNA ORF Clone, Mouse, C-HA tag MG50240-CY
    BDNF cDNA ORF Clone, Mouse, N-HA tag MG50240-NY
    BDNF cDNA ORF Clone, Mouse, C-Myc tag MG50240-CM
    BDNF cDNA ORF Clone, Mouse, N-Myc tag MG50240-NM
    BDNF qPCR Primer Pairs, Mouse MP200266

    BDNF cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    BDNF cDNA ORF Clone in Cloning Vector, Human HG10068-M
    BDNF cDNA ORF Clone, Human, untagged HG10068-UT
    BDNF cDNA ORF Clone, Human, C-GFPSpark® tag HG10068-ACG
    BDNF Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10068-ACGLN
    BDNF cDNA ORF Clone, Human, N-GFPSpark® tag HG10068-ANG
    BDNF cDNA ORF Clone, Human, C-OFPSpark® tag HG10068-ACR
    BDNF cDNA ORF Clone, Human, N-OFPSpark® tag HG10068-ANR
    BDNF cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10068-CF
    BDNF cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10068-NF
    BDNF cDNA ORF Clone, Human, C-His tag HG10068-CH
    BDNF cDNA ORF Clone, Human, N-His tag HG10068-NH
    BDNF cDNA ORF Clone, Human, C-HA tag HG10068-CY
    BDNF cDNA ORF Clone, Human, N-HA tag HG10068-NY
    BDNF cDNA ORF Clone, Human, C-Myc tag HG10068-CM
    BDNF cDNA ORF Clone, Human, N-Myc tag HG10068-NM
    BDNF qPCR Primer Pairs, Human HP100154
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