DPYS cDNA ORF Clone, Canine, C-DDK (Flag®) tag

Cat: DG70036-CF
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DPYS cDNA ORF Clone, Canine, C-DDK (Flag®) tag 基本信息
基因
种属
Canine
NCBI 参考序列号
参考序列ORF长度
1557 bp
描述
Full length Clone DNA of Canine dihydropyrimidinase-like with C terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-C-FLAG
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

DPYS Background Information

DPYS, also known as dihydropyrimidinase, belongs to the DHOase family, hydantoinase/dihydropyrimidinase subfamily. DPYS catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. It can catalyzes the ring opening of 5,6-dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate. DPYS is expressed at a high level in liver and kidney as a major 2.5-kb transcript and a minor 3.8-kb transcript. Defects in the DPYS gene are linked to dihydropyrimidinuria.

Full Name
dihydropyrimidinase
References
  • Thomas HR. et al., 2008, Genomics. 18 (1): 25-35.
  • Thomas HR. et al., 2008, Pharmacogenet Genomics. 17 (11): 973-87.
  • Van Kuilenburg AB. et al., 2007, Mol Genet Metab. 91 (2): 157-64.
  • DPYS cDNA ORF Clone, Canine, Related Products

    Product Name Catalog#(PDF)
    DPYS cDNA ORF Clone in Cloning Vector, Canine DG70036-G
    DPYS cDNA ORF Clone, Canine, untagged DG70036-UT
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    DPYS cDNA ORF Clone, Canine, C-His tag DG70036-CH
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    DPYS cDNA ORF Clone, Canine, C-HA tag DG70036-CY
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    DPYS cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    DPYS cDNA ORF Clone in Cloning Vector, Mouse MG53618-U
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    DPYS cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG53618-CF
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    DPYS cDNA ORF Clone, Mouse, C-His tag MG53618-CH
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    DPYS cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    DPYS cDNA ORF Clone in Cloning Vector, Human HG14884-G
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    DPYS cDNA ORF Clone, Human, N-GFPSpark® tag HG14884-ANG
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    DPYS cDNA ORF Clone, Human, C-His tag HG14884-CH
    DPYS cDNA ORF Clone, Human, N-His tag HG14884-NH
    DPYS cDNA ORF Clone, Human, C-HA tag HG14884-CY
    DPYS cDNA ORF Clone, Human, N-HA tag HG14884-NY
    DPYS cDNA ORF Clone, Human, C-Myc tag HG14884-CM
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