|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|狗 EGFR/HER1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||DG70026-ACG|
|狗 EGFR/HER1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||DG70026-ACR|
|狗 EGFR/HER1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||DG70026-CF|
|狗 EGFR/HER1 基因ORF全长cDNA克隆(表达载体), C-His 标签||DG70026-CH|
|狗 EGFR/HER1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||DG70026-CM|
|狗 EGFR/HER1 基因ORF全长cDNA克隆(表达载体), C-HA 标签||DG70026-CY|
|狗 EGFR/HER1 基因ORF全长cDNA(克隆载体)||DG70026-G|
|狗 EGFR/HER1 基因ORF全长cDNA克隆(表达载体), C-His 标签||DG70026-G-H|
|狗 EGFR/HER1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||DG70026-NF|
|狗 EGFR/HER1 基因ORF全长cDNA克隆(表达载体), N-His 标签||DG70026-NH|
|狗 EGFR/HER1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||DG70026-NM|
|狗 EGFR/HER1 基因ORF全长cDNA克隆(表达载体), N-HA 标签||DG70026-NY|
|狗 EGFR/HER1 基因ORF全长cDNA克隆(表达载体)||DG70026-UT|
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.