输入关键词搜索之后,可以在左侧选择分子或者产品类型以精简过滤器,快速查找产品。如果有任何问题,请 联系我们!

快速订购

Text Size:AAA

狗 IL1B 基因全长ORF克隆 (表达载体), C端FLAG标签

产品数据评论相关产品实验方法
Canine IL1B cDNA Clone产品信息
NCBI注册码:NM_001037971.1
参考序列ORF长度:798bp
cDNA基因描述:Full length Clone DNA of Canis lupus familiaris interleukin 1, beta with C terminal Flag tag.
分子别称:IL-1, IL1B
分子种属:Canine
载体:pCMV3-C-FLAG
质粒:
限制性酶切位点:
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Product nameProduct name
重组狗 IL1R2 / IL1RB 蛋白 (Fc 标签)重组大鼠 IL18R1 蛋白 (His 标签)重组小鼠 IL36G / IL1F9 蛋白 (His 标签)重组食蟹猴 IL18R1 蛋白 (Fc 标签)重组兔 IL-1 beta / IL1B 蛋白Human IL18 / IL-18 ProteinCanine IL18R1 Protein (ECD, His Tag)Human IL1RL2 / IL-1Rrp2 Protein (Fc Tag)重组食蟹猴 IL18R1 蛋白 (His 标签)重组人 ERK2 / MAPK1 / MAPK2 蛋白 (GST 标签)重组人 p38 alpha / MAPK14 蛋白 (His 标签)重组人 p38 alpha / MAPK14 蛋白 (His 标签)重组人 IL1RL1 / DER4 蛋白 (Fc 标签)重组人 IL1RL1 / ST2 蛋白 (His 标签)重组人 IL1RL1 / DER4 蛋白重组人 IL1R2 / CD121b 蛋白 (Fc 标签)重组人 IL1R2 / IL1RB / CD121b 蛋白 (His 标签)重组人 IL1R2 / IL1RB / CD121b 蛋白重组人 IL18 / Interleukin 18 / IGIF 蛋白 (GST 标签)重组人 IL-1RAcP / IL-1R3 蛋白 (His & Fc 标签)重组人 IL-1RAcP / IL-1R3 蛋白 (His 标签)重组人 IL-1RA / IL1RN 蛋白 (Fc 标签)重组人 IL-1RA / IL1RN 蛋白重组人 IL36G / IL1F9 蛋白 (aa 18-169, His 标签)重组人 IL36G / IL1F9 蛋白重组人 IL36G / IL1F9 蛋白 (aa 18-169)重组人 IL1F5 / IL36RN 蛋白重组人 IL1R1 / CD121a 蛋白 (Fc 标签)重组人 IL1R1 / CD121a 蛋白 (His 标签)重组人 IL1R1 / CD121a 蛋白重组人 IL-1 alpha / IL1A / IL1F1 蛋白重组人 IL-1 beta / IL1B 蛋白 (pro form, His 标签)重组人 IL-1 beta / IL1B 蛋白重组人 IL37 / IL1F7 / IL-1H4 蛋白 重组人 IL-1R9 / IL1RAPL2 蛋白 (Fc 标签)重组人 IL-1R9 / IL1RAPL2 蛋白 (His 标签)重组人 IL18RAP / IL1R7 蛋白 (Fc 标签)重组人 IL18RAP / IL1R7 蛋白 (His 标签)重组人 IL-1R8 / IL1RAPL1 蛋白 (Fc 标签)重组人 IL-1R8 / IL1RAPL1 蛋白 (His 标签)重组人 IL18BPa 蛋白 (His & Fc 标签)重组人 IL18BPa 蛋白 (His 标签)重组人 IL33 / Interleukin-33 / NF-HEV 蛋白重组人 MKK6 / MEK6 / MAP2K6 蛋白 (His & GST 标签)重组人 MKK6 / MEK6 / MAP2K6 蛋白 (207 Ser/Asp, 211 Thr/Asp, His & GST 标签)重组人 MKK6 / MEK6 / MAP2K6 蛋白重组人 MKK6 / MEK6 / MAP2K6 蛋白 (207 Asp, 211 Asp)重组人 IL36B / IL1F8 蛋白 (His 标签)重组人 IL36B / IL1F8 蛋白重组人 IL1F6 / IL36A 蛋白 (His 标签)重组人 IL1F6 / IL36A 蛋白重组人 IL1F6 / IL36 蛋白 (aa 6-158)重组小鼠 IL18RAP / IL1R7 蛋白 (His 标签)重组野猪 IL1B / IL-1 beta 蛋白重组人 JNK2 / MAPK9 蛋白 (His 标签)重组人 p38 delta / MAPK13 蛋白 (GST 标签)重组人 JNK1 / MAPK8 蛋白 (GST 标签)重组人 IL18R1 / CD218a 蛋白 (His & Fc 标签)重组人 IL18R1 / CD218a 蛋白 (His 标签)Human IL1RL2 / IL-1Rrp2 Protein (His Tag)重组人 IKB alpha / NFKBIA 蛋白 (His 标签)重组人 RELA / Transcription factor p65 / NFkB p65 蛋白 (aa 1-306, GST 标签)重组人 SIGIRR / TIR8 蛋白 (Fc 标签)重组人 SIGIRR / TIR8 蛋白 (His 标签)重组人 MARK3 / CTAK1 / EMK-2 蛋白 (His & GST 标签)重组猫 IL1B / IL-1 beta 蛋白重组人 IL1RL1 / ST2 蛋白 (isoform a, His 标签)重组人 p38 delta / MAPK13 蛋白 (Activated in vitro, GST 标签)重组小鼠 IL18 / IL-18 蛋白重组小鼠 IL-18R1 蛋白 (His & Fc 标签)重组小鼠 IL18R1 / CD218a 蛋白 (His 标签)重组小鼠 IL-1F6 / IL-1 epsilon 蛋白重组小鼠 IL-1 beta / IL1B 蛋白重组小鼠 IL-1 alpha / IL1A / IL1F1 蛋白重组小鼠 SIGIRR / TIR8 蛋白 (His & Fc 标签)重组小鼠 IL18BP 蛋白 (His 标签)重组小鼠 ERK2 / MAPK1 / MAPK2 蛋白 (His & GST 标签)重组小鼠 ERK2 / MAPK1 / MAPK2 蛋白重组小鼠 MKK4 / MEK4 / MAP2K4 蛋白 (His & GST 标签)重组小鼠 IL1RL1 / ST2 蛋白 (Fc 标签)重组小鼠 IL1R1 / CD121a 蛋白 (Fc 标签)重组小鼠 IL1R1 / CD121a 蛋白 (His 标签)重组小鼠 IL-1R8 / IL1RAPL1 蛋白 (Fc 标签)重组小鼠 IL1F8 / IL36b 蛋白 重组小鼠 IL1R2 / CD121b 蛋白 (Fc 标签)重组小鼠 IL1R2 / CD121b 蛋白 (His 标签)重组狗 IL33 / Interleukin-33 / NF-HEV 蛋白重组狗 IL-1 beta / IL1B 蛋白重组大鼠 IL-1 beta / IL1B 蛋白 (pro form, His 标签)重组大鼠 IL-1 beta / IL1B 蛋白 (mature form)重组大鼠 IL1R1 / CD121a 蛋白 (His & Fc 标签)重组大鼠 IL1R1 / CD121a 蛋白 (His 标签)重组大鼠 IL-1RA / IL1RN 蛋白 (Fc 标签)重组大鼠 IL18R1 蛋白 (Fc 标签)重组大鼠 IL1R2 / IL1RB / CD121b 蛋白 (His 标签)重组小鼠 IL1RL1 / ST2 蛋白 (His 标签)重组食蟹猴 IL-1 beta / IL1B 蛋白重组食蟹猴 IL-18 / IL-1F4 蛋白 (His 标签)重组食蟹猴 IL1R2 / IL1RB 蛋白 (Fc 标签)重组食蟹猴 IL18RAP 蛋白 (Fc 标签)重组食蟹猴 IL18RAP 蛋白 (His 标签)重组食蟹猴 IL1R1 蛋白 (Fc 标签)重组食蟹猴 IL1R1 蛋白 (His 标签)重组人 IL1F10 / IL-38 蛋白 (His 标签)重组狗 IL18R1 蛋白 (Fc 标签)
研究背景

Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc).

参考资料
  • Kim SH, et al. (2008) Association of -31TC and -511 CT polymorphisms in the interleukin 1 beta (IL1B) promoter in Korean keratoconus patients. Mol Vis. 14:2109-16.
  • Wang ZC, et al. (2002) T helper 1-type immunity to trophoblast antigens in women with a history of recurrent pregnancy loss is associated with polymorphism of the IL1B promoter region. Genes Immun. 3(1): 38-42.
  • Mattuzzi S, et al. (2007) Association of polymorphisms in the IL1B and IL2 genes with susceptibility and severity of systemic sclerosis. J Rheumatol. 34(5): 997-1004.
  • 产品数据可能随批次变化,最新数据请详见随货说明书。
    请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”