|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|狗 IL6 基因全长ORF克隆 (表达载体), C端GFPSpark标签||DG70015-ACG|
|狗 IL6 基因全长ORF克隆 (表达载体), C端OFPSpark标签||DG70015-ACR|
|狗 IL6 基因全长ORF克隆 (表达载体), C端FLAG标签||DG70015-CF|
|狗 IL6 基因全长ORF克隆 (表达载体), C端His标签||DG70015-CH|
|狗 IL6 基因全长ORF克隆 (表达载体), C端Myc标签||DG70015-CM|
|狗 IL6 基因全长ORF克隆 (表达载体), C端HA标签||DG70015-CY|
|狗 IL6 基因全长ORF克隆||DG70015-G|
|狗 IL6 基因全长ORF克隆 (表达载体), N端FLAG标签||DG70015-NF|
|狗 IL6 基因全长ORF克隆 (表达载体), N端His标签||DG70015-NH|
|狗 IL6 基因全长ORF克隆 (表达载体), N端Myc标签||DG70015-NM|
|狗 IL6 基因全长ORF克隆 (表达载体), N端HA标签||DG70015-NY|
|狗 IL6 基因全长ORF克隆 (表达载体), 无标签||DG70015-UT|
Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.