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狗 IL6 基因全长ORF克隆 (表达载体), C端FLAG标签

产品数据评论相关产品实验方法
Canine IL6 cDNA Clone产品信息
NCBI注册码:NM_001003301.1
参考序列ORF长度:624bp
cDNA基因描述:Full length Clone DNA of Canis lupus familiaris interleukin 6 (interferon, beta 2) with C terminal Flag tag.
分子别称:IL-6, IL6
分子种属:Canine
载体:pCMV3-C-FLAG
质粒:
限制性酶切位点:
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Product nameProduct name
重组大鼠 GM-CSF / CSF2 蛋白 (Fc 标签)重组小鼠 Oncostatin M / OSM 蛋白重组人 Interleukin-31 receptor A / IL31RA 蛋白 (Fc 标签, ECD)Canine IL-6R / CD126 Protein (ECD, His Tag)重组人 Oncostatin M / OSM 蛋白重组狗 IL11RA / IL-11RA 蛋白 (Fc 标签)重组人 G-CSF / CSF3 蛋白 (isoform b)Rat IL-11RA1 / Il11RA1 Protein (His Tag)重组小鼠 CNTF / Ciliary Neurotrophic Factor 蛋白 (His 标签)重组人 NNT1 / CLCF1 / CLC 蛋白 (Fc 标签)重组人 G-CSF / CSF3 蛋白 (Fc 标签)重组人 GM-CSF / CSF2 蛋白 (Fc 标签)重组人 GM-CSF / CSF2 蛋白 (His 标签)重组人 G-CSFR / CD114 / CSF3R 蛋白 (Fc 标签)重组人 G-CSFR / CD114 蛋白 (His 标签)重组人 G-CSFR / CD114 / CSF3R 蛋白重组人 Leptin 蛋白重组人 IL11RA / IL11Rα 蛋白 (His 标签)重组人 Leptin Receptor / LEPR / CD295 蛋白 (His & Fc 标签)重组人 Leptin Receptor / LEPR / CD295 蛋白 (His 标签)重组人 IL6 / Interleukin-6 蛋白重组人 IL6R / CD126 蛋白 (His 标签)重组人 Oncostatin M / OSM 蛋白 (His 标签)重组人 LIFR / CD118 蛋白 (His 标签)重组人 CSF2RA / GM-CSFR / CD116 蛋白 (Fc 标签)重组人 CSF2RA / GM-CSFR / CD116 蛋白 (His 标签)重组人 IL6ST / gp130 / CD130 蛋白 (His & Fc 标签)重组人 IL6ST / gp130 / CD130 蛋白重组人 CNTFR / CNTFR-alpha 蛋白 (His 标签)重组人 OSMR / IL31RB 蛋白 (His 标签)重组小鼠 IL-31 / IL31 蛋白 (His 标签)重组人 CNTF 蛋白 (His 标签)重组人 IL11 / Interleukin 11 / IL-11 蛋白重组大鼠 IL-6R / CD126 蛋白 (ECD, His 标签)重组人 G-CSF / CSF3 蛋白 (isoform b)重组人 LIF 蛋白 (Fc 标签)重组人 LIF 蛋白 (His 标签)重组小鼠 IL11RA / IL11Rα 蛋白 (His 标签)重组小鼠 Oncostatin M / OSM 蛋白 (His 标签)重组小鼠 IL6ST / CD130 蛋白 (His & Fc 标签)重组小鼠 IL6ST / gp130 / CD130 蛋白 (His & Fc 标签)重组小鼠 IL6ST / gp130 / CD130 蛋白 (His 标签)重组小鼠 IL6 / Interleukin-6 蛋白重组小鼠 IL6RA / CD126 蛋白 (His 标签)重组小鼠 LIFR / CD118 蛋白 (His 标签)重组小鼠 OSMR / IL-31RB 蛋白 (His 标签)重组小鼠 GM-CSF / CSF2 蛋白 (Fc 标签)重组小鼠 GM-CSF / CSF2 蛋白 (His 标签)重组人 GM-CSF / CSF2 蛋白重组大鼠 CNTF / Ciliary Neurotrophic Factor 蛋白重组大鼠 CNTFR / CNTFR-alpha 蛋白 (His 标签)重组大鼠 GM-CSF / CSF2 蛋白 (His 标签)重组大鼠 gp130 / IL6ST / CD130 蛋白 (His & Fc 标签)重组大鼠 gp130 / IL6ST / CD130 蛋白 (His 标签)重组人 GM-CSF / CSF2 蛋白重组大鼠 IL6 / Interleukin-6 蛋白重组狗 IL11RA / IL-11RA / IL11Rα 蛋白 (His 标签)重组大鼠 LIFR 蛋白 (His 标签)重组人 LIF 蛋白重组人 IL-31 / IL31 蛋白 (His 标签)重组食蟹猴 / 恒河猴 IL6 / Interleukin-6 蛋白重组食蟹猴 IL6ST / gp130 蛋白 (Fc 标签)重组食蟹猴 IL6ST / gp130 蛋白 (His 标签)重组小鼠 GM-CSF / CSF2 蛋白重组小鼠 IL-11 / interleukin 11 蛋白重组野猪 IL6 / IL-6 蛋白重组大鼠 IL-6R / CD126 蛋白 (Fc 标签, ECD)Mouse LIF Protein重组人 Interleukin-31 receptor A / IL31RA 蛋白 (His 标签)重组人 IL6ST / gp130 / CD130 蛋白 (ECD, Fc Tag)重组狗 IL11RA / IL-11RA / IL11Rα 蛋白
研究背景

Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

参考资料
  • Heinrich PC. et al. (2003). Principles of interleukin-6-type cytokine signalling and its regulation. Biochem J. 374: 1-20.
  • Rose-John S, et al. (2007) The IL-6/sIL-6R complex as a novel target for therapeutic approaches. Expert Opin Ther Targets. 11(5): 613-24.
  • Dinh W, et al. (2009) Elevated plasma levels of TNF-alpha and interleukin-6 in patients with diastolic dysfunction and glucose metabolism disorders. Cardiovasc Diabetol. 8:58.
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    货号: DG70015-CF
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