|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.
|狗 NME1/NDKA 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||DG70213-ACG|
|狗 NME1/NDKA 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||DG70213-ACR|
|狗 NME1/NDKA 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||DG70213-ANG|
|狗 NME1/NDKA 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||DG70213-ANR|
|狗 NME1/NDKA 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||DG70213-CF|
|狗 NME1/NDKA 基因ORF全长cDNA克隆(表达载体), C-His 标签||DG70213-CH|
|狗 NME1/NDKA 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||DG70213-CM|
|狗 NME1/NDKA 基因ORF全长cDNA克隆(表达载体), C-HA 标签||DG70213-CY|
|狗 NME1/NDKA 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||DG70213-NF|
|狗 NME1/NDKA 基因ORF全长cDNA克隆(表达载体), N-His 标签||DG70213-NH|
|狗 NME1/NDKA 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||DG70213-NM|
|狗 NME1/NDKA 基因ORF全长cDNA克隆(表达载体), N-HA 标签||DG70213-NY|
|狗 NME1/NDKA 基因ORF全长cDNA(克隆载体)||DG70213-U|
|狗 NME1/NDKA 基因ORF全长cDNA克隆(表达载体)||DG70213-UT|
NME1, also known as Nucleoside Diphosphate Kinase A (NDK-A), or NM23-H1, belongs to the NDK family. NM23-H1 is known to have a metastasis suppressive activity in many tumor cells. Recent studies have shown that the interacting proteins with NM23-H1 which mediate the cell proliferation, may act as modulators of the metastasis suppressor activity. The interacting proteins with NM23-H1 can be classified into 3 groups. The first group of proteins can be classified as upstream kinases of NM23-H1 such as CKI and Aurora-A/STK15. The second group of proteins acts as downstream effectors for the regulation of specific gene transcriptions, GTP-binding protein functions, and signal transduction in Erk signal cascade. The third group of proteins can be classified as bi-directionally influencing binding partners of NM23-H1. As a result, the interactions with NM23-H1 and binding partners have implications in the biochemical characterization involved in metastasis and tumorigenesis. NDKA is increased in human postmortem cerebrospinal fluid (CSF), a model of global brain insult, suggesting that measurement in CSF and, more importantly, in plasma may be useful as a biomarker of stroke. Additionally, NM23-H1 significantly reduces metastasis without effects on primary tumor size and was the first discovered metastasis suppressor gene.