Anti-CA9 rabbit monoclonal antibody at 1:500 dilution
Lane A: A431 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:50 kDa
Observed band size:40 kDa
CA9 was immunoprecipitated using:
Lane A:0.5 mg A431 Whole Cell Lysate2 µL anti-CA9 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-CA9 rabbit monoclonal antibody,at 1:200 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 40 kDa
Observed band size: 40 kDa